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Chacón-Duque, J. C., Thomas Thorpe, J. A., Li, W., Dehasque, M., Pečnerová, P., Barlow, A., . . . Dalén, L. (2025). A Million Years of Mammoth Mitogenome Evolution. Molecular biology and evolution, 42(4), Article ID msaf065.
Öppna denna publikation i ny flik eller fönster >>A Million Years of Mammoth Mitogenome Evolution
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2025 (Engelska)Ingår i: Molecular biology and evolution, ISSN 0737-4038, E-ISSN 1537-1719, Vol. 42, nr 4, artikel-id msaf065Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The genomic study of specimens dating to the Early and Middle Pleistocene (EP and MP), a period spanning from 2.6 million years ago (Ma) to 126 thousand years ago (ka), has the potential to elucidate the evolutionary processes that shaped present-day biodiversity. Obtaining genomic data from this period is challenging, but mitochondrial DNA, given its higher abundance compared to nuclear DNA, could play an important role to understand evolutionary processes at this time scale. In this study, we report 34 new mitogenomes, including two EP and nine MP mammoth (Mammuthus spp.) specimens from Siberia and North America and analyze them jointly with >200 publicly available mitogenomes to reconstruct a transect of mammoth mitogenome diversity throughout the last million years. We find that our EP mitogenomes fall outside the diversity of all Late Pleistocene (LP) mammoths, while those derived from MP mammoths are basal to LP mammoth Clades 2 and 3, supporting an ancient Siberian origin of these lineages. In contrast, the geographical origin of Clade 1 remains unresolved. With these new deep-Time mitogenomes, we observe diversification events across all clades that appear consistent with previously hypothesized MP and LP demographic changes. Furthermore, we improve upon an existing methodology for molecular clock dating of specimens >50 ka, demonstrating that specimens need to be individually dated to avoid biases in their age estimates. Both the molecular and analytical improvements presented here highlight the importance of deep-Time genomic data to discover long-lost genetic diversity, enabling better assessments of evolutionary histories.

Nyckelord
deep-Time DNA, mammoths, mitogenomes, molecular clock dating, palaeogenomics, phylogenetics
Nationell ämneskategori
Zoologi Genetik och genomik
Identifikatorer
urn:nbn:se:su:diva-242989 (URN)10.1093/molbev/msaf065 (DOI)001463164300001 ()40202893 (PubMedID)2-s2.0-105002791877 (Scopus ID)
Tillgänglig från: 2025-05-08 Skapad: 2025-05-08 Senast uppdaterad: 2025-05-08Bibliografiskt granskad
Dehasque, M., Morales, H. E., Díez-del-Molino, D., Pečnerová, P., Chacón-Duque, J. C., Kanellidou, F., . . . Dalén, L. (2024). Temporal dynamics of woolly mammoth genome erosion prior to extinction. Cell, 187(14), p3531-3540.e13
Öppna denna publikation i ny flik eller fönster >>Temporal dynamics of woolly mammoth genome erosion prior to extinction
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2024 (Engelska)Ingår i: Cell, ISSN 0092-8674, E-ISSN 1097-4172, Vol. 187, nr 14, s. p3531-3540.e13Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A number of species have recently recovered from near-extinction. Although these species have avoided the immediate extinction threat, their long-term viability remains precarious due to the potential genetic consequences of population declines, which are poorly understood on a timescale beyond a few generations. Woolly mammoths (Mammuthus primigenius) became isolated on Wrangel Island around 10,000 years ago and persisted for over 200 generations before becoming extinct around 4,000 years ago. To study the evolutionary processes leading up to the mammoths’ extinction, we analyzed 21 Siberian woolly mammoth genomes. Our results show that the population recovered quickly from a severe bottleneck and remained demographically stable during the ensuing six millennia. We find that mildly deleterious mutations gradually accumulated, whereas highly deleterious mutations were purged, suggesting ongoing inbreeding depression that lasted for hundreds of generations. The time-lag between demographic and genetic recovery has wide-ranging implications for conservation management of recently bottlenecked populations.

Nyckelord
ancient DNA, bottleneck, climate, extinction, inbreeding, Mammuthus primigenius, mutation load, paleogenomics, woolly mammoth, Wrangel Island
Nationell ämneskategori
Zoologi
Identifikatorer
urn:nbn:se:su:diva-238305 (URN)10.1016/j.cell.2024.05.033 (DOI)38942016 (PubMedID)2-s2.0-85197516018 (Scopus ID)
Tillgänglig från: 2025-01-23 Skapad: 2025-01-23 Senast uppdaterad: 2025-01-23Bibliografiskt granskad
Sandoval-Velasco, M., Dehasque, M., Dalén, L. & Aiden, E. L. (2024). Three-dimensional genome architecture persists in a 52,000-year-old woolly mammoth skin sample. Cell, 187(14), P3541-3562.e51
Öppna denna publikation i ny flik eller fönster >>Three-dimensional genome architecture persists in a 52,000-year-old woolly mammoth skin sample
2024 (Engelska)Ingår i: Cell, ISSN 0092-8674, E-ISSN 1097-4172, Vol. 187, nr 14, s. P3541-3562.e51Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Analyses of ancient DNA typically involve sequencing the surviving short oligonucleotides and aligning to genome assemblies from related, modern species. Here, we report that skin from a female woolly mammoth (†Mammuthus primigenius) that died 52,000 years ago retained its ancient genome architecture. We use PaleoHi-C to map chromatin contacts and assemble its genome, yielding 28 chromosome-length scaffolds. Chromosome territories, compartments, loops, Barr bodies, and inactive X chromosome (Xi) superdomains persist. The active and inactive genome compartments in mammoth skin more closely resemble Asian elephant skin than other elephant tissues. Our analyses uncover new biology. Differences in compartmentalization reveal genes whose transcription was potentially altered in mammoths vs. elephants. Mammoth Xi has a tetradic architecture, not bipartite like human and mouse. We hypothesize that, shortly after this mammoth's death, the sample spontaneously freeze-dried in the Siberian cold, leading to a glass transition that preserved subfossils of ancient chromosomes at nanometer scale.

Nyckelord
ancient DNA, chromatin loops, fossil, genome architecture, genome assembly, glass transition, Hi-C, vitrification, woolly mammoth, X inactivation
Nationell ämneskategori
Genetik och genomik
Identifikatorer
urn:nbn:se:su:diva-238304 (URN)10.1016/j.cell.2024.06.002 (DOI)38996487 (PubMedID)2-s2.0-85197549522 (Scopus ID)
Tillgänglig från: 2025-01-23 Skapad: 2025-01-23 Senast uppdaterad: 2025-01-23Bibliografiskt granskad
Díez-del-Molino, D., Dehasque, M., Chacón-Duque, J. C., Pecnerova, P., Tikhonov, A., Protopopov, A., . . . Dalén, L. (2023). Genomics of adaptive evolution in the woolly mammoth. Current Biology, 33(9), 1753-1764
Öppna denna publikation i ny flik eller fönster >>Genomics of adaptive evolution in the woolly mammoth
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2023 (Engelska)Ingår i: Current Biology, ISSN 0960-9822, E-ISSN 1879-0445, Vol. 33, nr 9, s. 1753-1764Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Ancient genomes provide a tool to investigate the genetic basis of adaptations in extinct organisms. However, the identification of species-specific fixed genetic variants requires the analysis of genomes from multiple individuals. Moreover, the long-term scale of adaptive evolution coupled with the short-term nature of tradi-tional time series data has made it difficult to assess when different adaptations evolved. Here, we analyze 23 woolly mammoth genomes, including one of the oldest known specimens at 700,000 years old, to identify fixed derived non-synonymous mutations unique to the species and to obtain estimates of when these mutations evolved. We find that at the time of its origin, the woolly mammoth had already acquired a broad spectrum of positively selected genes, including ones associated with hair and skin development, fat storage and metabolism, and immune system function. Our results also suggest that these phenotypes continued to evolve during the last 700,000 years, but through positive selection on different sets of genes. Finally, we also identify additional genes that underwent comparatively recent positive selection, including multiple genes related to skeletal morphology and body size, as well as one gene that may have contributed to the small ear size in Late Quaternary woolly mammoths.

Nationell ämneskategori
Evolutionsbiologi
Identifikatorer
urn:nbn:se:su:diva-230740 (URN)10.1016/j.cub.2023.03.084 (DOI)001007407600001 ()37030294 (PubMedID)2-s2.0-85153039198 (Scopus ID)
Tillgänglig från: 2024-06-11 Skapad: 2024-06-11 Senast uppdaterad: 2024-06-11Bibliografiskt granskad
Dehasque, M. (2023). Palaeogenomic reconstruction of woolly mammoth evolutionary history and extinction dynamics. (Doctoral dissertation). Stockholm: Department of Zoology, Stockholm University
Öppna denna publikation i ny flik eller fönster >>Palaeogenomic reconstruction of woolly mammoth evolutionary history and extinction dynamics
2023 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Biodiversity is declining globally. Yet, the biological and genetic processes associated with these declines on a longer timescale are still poorly understood. Ancient DNA is a powerful tool to study evolution in real-time. Despite advances in the field, there is further need for refinement of laboratory and computational techniques. In this thesis, I used mitochondrial and nuclear genomes, as well as radiocarbon data, to study the evolutionary history and extinction dynamics of the woolly mammoth (Mammuthus primigenius). In Chapter I, I developed and optimized a silica column-based extraction protocol for ancient DNA. Based on systematic tests, I advise against routine use of pretreatment methods, like bleach wash and/or predigestion, for well-preserved permafrost samples. Furthermore, I suggest that USER enzyme, which removes uracil from damaged DNA molecules, is effective at half the concentration compared to an established control protocol. Finally, I did not find a significant difference between different silica columns for the clean-up steps, or concentrator columns with different DNA retention sizes. In Chapter II, I used five high coverage Siberian mammoth genomes to develop a method based on differences in read depth to identify indels, insertions and deletions, in the mammoth genome. The results show that indels are enriched in intergenic regions, suggesting strong selection against structural variants affecting gene function. Nevertheless, 87 genes were identified that were severely affected. These genes are related to various functions like body-fat distribution, fur growth and hair shape, body temperature, and body size, and most likely represent important adaptations to the cold steppe-tundra. In Chapter III, I studied the population and extinction dynamics of the woolly mammoths in Siberia by combining Bayesian age models from radiocarbon data with inferences from complete mitogenomes. The results show that the woolly mammoth’s extinction was a complex process with consecutive extirpations, but also partial recolonizations, occurring in different Siberian localities. I hypothesize that Wrangel Island, one of the last refugia of the woolly mammoth, was colonized by mammoths from, or closely related to, a population from central or western Siberia. Mammoths reappeared on the island around 10 ky ago, where they became isolated due to rising sea levels, and went extinct around 4 ky ago. To investigate the genetic consequences of the founder bottleneck event of Wrangel Island and long-term survival at small population size, I analyzed time-series data of 21 woolly mammoth genomes in Chapter IV. Changes in heterozygosity and inbreeding show that while the initial bottleneck was dramatic, the population recovered fast and remained remarkably stable. Analysis of mutation load shows that mammoths purged highly deleterious mutations over time, but also accumulated slightly deleterious mutations, indicating reduced efficacy of selection. Nevertheless, there were no clear signs of a mutational meltdown, and it remains a mystery why mammoths went extinct on the island. In conclusion, this thesis presents methodological advances for DNA extraction and detecting structural variants (i.e., deletions) in ancient genomes. Furthermore, I demonstrate that extinctions are a complex process. Finally, I show that ancient DNA is a powerful tool to study evolutionary processes over long timescales.

Ort, förlag, år, upplaga, sidor
Stockholm: Department of Zoology, Stockholm University, 2023. s. 42
Nyckelord
woolly mammoth, Mammuthus primigenius, extinction, Wrangel Island, ancient DNA, palaeogenomics, radiocarbon dating, structural variants, genome erosion, population structure
Nationell ämneskategori
Biologiska vetenskaper
Forskningsämne
zoologisk systematik och evolutionsforskning
Identifikatorer
urn:nbn:se:su:diva-214156 (URN)978-91-8014-176-5 (ISBN)978-91-8014-177-2 (ISBN)
Disputation
2023-03-10, Vivi Täckholmsalen (Q-salen), NPQ-huset, Svante Arrhenius väg 20, Stockholm, 10:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2023-02-15 Skapad: 2023-01-25 Senast uppdaterad: 2023-02-06Bibliografiskt granskad
Dehasque, M., Pečnerová, P., Kempe Lagerholm, V., Ersmark, E., Danilov, G. K., Mortensen, P., . . . Dalén, L. (2022). Development and Optimization of a Silica Column-Based Extraction Protocol for Ancient DNA. Genes, 13(4), Article ID 687.
Öppna denna publikation i ny flik eller fönster >>Development and Optimization of a Silica Column-Based Extraction Protocol for Ancient DNA
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2022 (Engelska)Ingår i: Genes, E-ISSN 2073-4425, Vol. 13, nr 4, artikel-id 687Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Rapid and cost-effective retrieval of endogenous DNA from ancient specimens remains a limiting factor in palaeogenomic research. Many methods have been developed to increase ancient DNA yield, but modifications to existing protocols are often based on personal experience rather than systematic testing. Here, we present a new silica column-based extraction protocol, where optimizations were tested in controlled experiments. Using relatively well-preserved permafrost samples, we tested the efficiency of pretreatment of bone and tooth powder with a bleach wash and a predigestion step. We also tested the recovery efficiency of MinElute and QIAquick columns, as well as Vivaspin columns with two molecular weight cut-off values. Finally, we tested the effect of uracil-treatment with two different USER enzyme concentrations. We find that neither bleach wash combined with a predigestion step, nor predigestion by itself, significantly increased sequencing efficiency. Initial results, however, suggest that MinElute columns are more efficient for ancient DNA extractions than QIAquick columns, whereas different molecular weight cut-off values in centrifugal concentrator columns did not have an effect. Uracil treatments are effective at removing DNA damage even at concentrations of 0.15 U/µL (as compared to 0.3 U/µL) of ancient DNA extracts.

Nyckelord
ancient DNA, woolly mammoth, DNA extraction, high-throughput sequencing, bone
Nationell ämneskategori
Biologiska vetenskaper
Identifikatorer
urn:nbn:se:su:diva-204673 (URN)10.3390/genes13040687 (DOI)000786976400001 ()35456493 (PubMedID)2-s2.0-85129129475 (Scopus ID)
Tillgänglig från: 2022-05-20 Skapad: 2022-05-20 Senast uppdaterad: 2024-07-04Bibliografiskt granskad
van der Valk, T., Dehasque, M., Chacón-Duque, J. C., Oskolkov, N., Vartanyan, S., Heintzman, P. D., . . . Dalén, L. (2022). Evolutionary consequences of genomic deletions and insertions in the woolly mammoth genome. iScience, 25(8), Article ID 104826.
Öppna denna publikation i ny flik eller fönster >>Evolutionary consequences of genomic deletions and insertions in the woolly mammoth genome
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2022 (Engelska)Ingår i: iScience, E-ISSN 2589-0042, Vol. 25, nr 8, artikel-id 104826Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Woolly mammoths had a set of adaptations that enabled them to thrive in the Arctic environment. Many mammoth-specific single nucleotide polymorphisms (SNPs) responsible for unique mammoth traits have been previously identified from ancient genomes. However, a multitude of other genetic variants likely contributed to woolly mammoth evolution. In this study, we sequenced two woolly mammoth genomes and combined these with previously sequenced mammoth and elephant genomes to conduct a survey of mammoth-specific deletions and indels. We find that deletions are highly enriched in non-coding regions, suggesting selection against structural variants that affect protein sequences. Nonetheless, at least 87 woolly mammoth genes contain deletions or indels that modify the coding sequence, including genes involved in skeletal morphology and hair growth. These results suggest that deletions and indels contributed to the unique phenotypic adaptations of the woolly mammoth, and were potentially critical to surviving in its natural environment. 

Nyckelord
Bioinformatics, Biological sciences, Evolutionary biology, Natural sciences, Phylogenetics, Zoology
Nationell ämneskategori
Biologiska vetenskaper
Identifikatorer
urn:nbn:se:su:diva-211994 (URN)10.1016/j.isci.2022.104826 (DOI)000995394500001 ()2-s2.0-85135699106 (Scopus ID)
Tillgänglig från: 2022-12-01 Skapad: 2022-12-01 Senast uppdaterad: 2024-06-04Bibliografiskt granskad
Kutschera, V. E., Kierczak, M., van der Valk, T., von Seth, J., Dussex, N., Lord, E., . . . Díez-del-Molino, D. (2022). GenErode: a bioinformatics pipeline to investigate genome erosion in endangered and extinct species. BMC Bioinformatics, 23(1), Article ID 228.
Öppna denna publikation i ny flik eller fönster >>GenErode: a bioinformatics pipeline to investigate genome erosion in endangered and extinct species
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2022 (Engelska)Ingår i: BMC Bioinformatics, E-ISSN 1471-2105, Vol. 23, nr 1, artikel-id 228Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Many wild species have suffered drastic population size declines over the past centuries, which have led to 'genomic erosion' processes characterized by reduced genetic diversity, increased inbreeding, and accumulation of harmful mutations. Yet, genomic erosion estimates of modern-day populations often lack concordance with dwindling population sizes and conservation status of threatened species. One way to directly quantify the genomic consequences of population declines is to compare genome-wide data from pre-decline museum samples and modern samples. However, doing so requires computational data processing and analysis tools specifically adapted to comparative analyses of degraded, ancient or historical, DNA data with modern DNA data as well as personnel trained to perform such analyses.

Results: Here, we present a highly flexible, scalable, and modular pipeline to compare patterns of genomic erosion using samples from disparate time periods. The GenErode pipeline uses state-of-the-art bioinformatics tools to simultaneously process whole-genome re-sequencing data from ancient/historical and modern samples, and to produce comparable estimates of several genomic erosion indices. No programming knowledge is required to run the pipeline and all bioinformatic steps are well-documented, making the pipeline accessible to users with different backgrounds. GenErode is written in Snakemake and Python3 and uses Conda and Singularity containers to achieve reproducibility on high-performance compute clusters. The source code is freely available on GitHub (https://github.com/NBISweden/GenErode).

Conclusions: GenErode is a user-friendly and reproducible pipeline that enables the standardization of genomic erosion indices from temporally sampled whole genome re-sequencing data.

Nyckelord
Bioinformatics, Temporal genomic erosion, Conservation genomics, Whole genome re-sequencing data, Endangered species, Reproducibility, Snakemake, Ancient DNA
Nationell ämneskategori
Biologiska vetenskaper
Identifikatorer
urn:nbn:se:su:diva-207253 (URN)10.1186/s12859-022-04757-0 (DOI)000810679500003 ()35698034 (PubMedID)2-s2.0-85131799948 (Scopus ID)
Tillgänglig från: 2022-07-18 Skapad: 2022-07-18 Senast uppdaterad: 2024-01-17Bibliografiskt granskad
Rosengren, E., Acatrinei, A., Cruceru, N., Dehasque, M., Haliuc, A., Lord, E., . . . Meleg, I. N. (2021). Ancient Faunal History Revealed by Interdisciplinary Biomolecular Approaches. Diversity, 13(8), Article ID 370.
Öppna denna publikation i ny flik eller fönster >>Ancient Faunal History Revealed by Interdisciplinary Biomolecular Approaches
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2021 (Engelska)Ingår i: Diversity, E-ISSN 1424-2818, Vol. 13, nr 8, artikel-id 370Artikel, forskningsöversikt (Refereegranskat) Published
Abstract [en]

Starting four decades ago, studies have examined the ecology and evolutionary dynamics of populations and species using short mitochondrial DNA fragments and stable isotopes. Through technological and analytical advances, the methods and biomolecules at our disposal have increased significantly to now include lipids, whole genomes, proteomes, and even epigenomes. At an unprecedented resolution, the study of ancient biomolecules has made it possible for us to disentangle the complex processes that shaped the ancient faunal diversity across millennia, with the potential to aid in implicating probable causes of species extinction and how humans impacted the genetics and ecology of wild and domestic species. However, even now, few studies explore interdisciplinary biomolecular approaches to reveal ancient faunal diversity dynamics in relation to environmental and anthropogenic impact. This review will approach how biomolecules have been implemented in a broad variety of topics and species, from the extinct Pleistocene megafauna to ancient wild and domestic stocks, as well as how their future use has the potential to offer an enhanced understanding of drivers of past faunal diversity on Earth.

Nyckelord
ancient DNA, wild and domestic ancient fauna, ancient genomics, ancient epigenomics, ancient proteomics, ancient lipidomics, stable isotopes, biomolecular archaeology and palaeontology, palaeoecology
Nationell ämneskategori
Biologiska vetenskaper
Identifikatorer
urn:nbn:se:su:diva-197504 (URN)10.3390/d13080370 (DOI)000688789000001 ()
Tillgänglig från: 2021-10-05 Skapad: 2021-10-05 Senast uppdaterad: 2024-01-30Bibliografiskt granskad
Dehasque, M., Pečnerová, P., Muller, H., Tikhonov, A., Nikolskiy, P., Tsigankova, V., . . . Lister, A. M. (2021). Combining Bayesian age models and genetics to investigate population dynamics and extinction of the last mammoths in northern Siberia. Quaternary Science Reviews, 259, Article ID 106913.
Öppna denna publikation i ny flik eller fönster >>Combining Bayesian age models and genetics to investigate population dynamics and extinction of the last mammoths in northern Siberia
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2021 (Engelska)Ingår i: Quaternary Science Reviews, ISSN 0277-3791, E-ISSN 1873-457X, Vol. 259, artikel-id 106913Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

To understand the causes and implications of an extinction event, detailed information is necessary. However, this can be challenging when working with poorly resolved paleontological data sets. One approach to increase the data resolution is by combining different methods. In this study, we used both radiocarbon and genetic data to reconstruct the population history and extinction dynamics of the woolly mammoth in northern Siberia. We generated 88 new radiocarbon dates and combined these with previously published dates from 626 specimens to construct Bayesian age models. These models show that mammoths disappeared on the eastern Siberian mainland before the onset of the Younger Dryas (12.9–11.7 ky cal BP). Mammoths did however persist in the northernmost parts of central and western Siberia until the early Holocene. Further genetic results of 131 high quality mitogenomes, including 22 new mitogenomes generated in this study, support the hypothesis that mammoths from, or closely related to, a central and/or west- Siberian population recolonized Wrangel Island over the now submerged northern Siberian plains. As mammoths became trapped on the island due to rising sea levels, they lived another ca. 6000 years on Wrangel Island before eventually going extinct ca. 4000 years ago.

Nyckelord
Ancient DNA, Radiocarbon, Woolly mammoth, Mitochondrial genomes, Bayesian age modelling
Nationell ämneskategori
Geovetenskap och relaterad miljövetenskap
Identifikatorer
urn:nbn:se:su:diva-195649 (URN)10.1016/j.quascirev.2021.106913 (DOI)000644423800009 ()
Tillgänglig från: 2021-08-24 Skapad: 2021-08-24 Senast uppdaterad: 2025-02-07Bibliografiskt granskad
Organisationer
Identifikatorer
ORCID-id: ORCID iD iconorcid.org/0000-0002-4640-8306

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