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Persson, Carina
Publications (2 of 2) Show all publications
Persson, C., Oldenvi, S. & Steiner, H. (2007). Peptidoglycan recognition protein LF, A negative regulator of Drosophila immunity.. Insect Biochem Mol Biol, 37(12), 1309-16
Open this publication in new window or tab >>Peptidoglycan recognition protein LF, A negative regulator of Drosophila immunity.
2007 (English)In: Insect Biochem Mol Biol, ISSN 0965-1748, Vol. 37, no 12, p. 1309-16Article in journal (Refereed) Published
Identifiers
urn:nbn:se:su:diva-20026 (URN)000251401800007 ()17967349 (PubMedID)
Available from: 2007-11-21 Created: 2007-11-21 Last updated: 2022-02-25Bibliographically approved
Gelius, E., Persson, C., Karlsson, J. & Steiner, H. (2003). A mammalian peptidoglycan recognition protein with N-acetylmuramoyl-L-alanine amidase activity. Biochemical and Biophysical Research Communications - BBRC, 306(4), 988-994
Open this publication in new window or tab >>A mammalian peptidoglycan recognition protein with N-acetylmuramoyl-L-alanine amidase activity
2003 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 306, no 4, p. 988-994Article in journal (Refereed) Published
Abstract [en]

The family of peptidoglycan recognition proteins (PGRPs) is conserved from insects to mammals. Recently, Drosophila PGRP-SC1B was demonstrated to be an N-acetylmuramoyl- -alanine amidase (NAMLAA), an enzyme that cleaves the lactylamide bond between muramic acid and the peptide chain in peptidoglycan (PGN). We now show an M.mPGRP-L mRNA to be expressed in the liver. The recombinant M.mPGRP-L protein has NAMLAA activity and degrades PGN from both Escherichia coli and Staphylococcus aureus; however, the Gram-positive PGN was a better substrate after lysozyme treatment. The activity of M.mPGRP-L was further analysed using Bordetella pertussis tracheal toxin as a substrate. Cleavage products were separated on HPLC and identified using mass spectrometry. From these results we conclude that M.mPGRP-L has activity and other properties identifying it as the NAMLAA protein present in mammalian sera.

Identifiers
urn:nbn:se:su:diva-20035 (URN)10.1016/S0006-291X(03)01096-9 (DOI)12821140 (PubMedID)
Available from: 2007-11-21 Created: 2007-11-21 Last updated: 2022-02-25Bibliographically approved
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