Change search
Link to record
Permanent link

Direct link
Singh, Abeer Prakash
Alternative names
Publications (6 of 6) Show all publications
Singh, A. P., Salvatori, R., Aftab, W., Aufschnaiter, A., Carlström, A., Forne, I., . . . Ott, M. (2020). Molecular Connectivity of Mitochondrial Gene Expression and OXPHOS Biogenesis. Molecular Cell, 79(6), 1051-1065
Open this publication in new window or tab >>Molecular Connectivity of Mitochondrial Gene Expression and OXPHOS Biogenesis
Show others...
2020 (English)In: Molecular Cell, ISSN 1097-2765, E-ISSN 1097-4164, Vol. 79, no 6, p. 1051-1065Article in journal (Refereed) Published
Abstract [en]

Mitochondria contain their own gene expression systems, including membrane-bound ribosomes dedicated to synthesizing a few hydrophobic subunits of the oxidative phosphorylation (OXPHOS) complexes. We used a proximity-dependent biotinylation technique, BiolD, coupled with mass spectrometry to delineate in baker's yeast a comprehensive network of factors involved in biogenesis of mitochondrial encoded proteins. This mitochondrial gene expression network (MiGENet) encompasses proteins involved in transcription, RNA processing, translation, or protein biogenesis. Our analyses indicate the spatial organization of these processes, thereby revealing basic mechanistic principles and the proteins populating strategically important sites. For example, newly synthesized proteins are directly handed over to ribosomal tunnel exit-bound factors that mediate membrane insertion, co-factor acquisition, or their mounting into OXPHOS complexes in a special early assembly hub. Collectively, the data reveal the connectivity of mitochondrial gene expression, reflecting a unique tailoring of the mitochondrial gene expression system.

National Category
Biological Sciences
Identifiers
urn:nbn:se:su:diva-187331 (URN)10.1016/j.molcel.2020.07.024 (DOI)000573535400006 ()32877643 (PubMedID)
Available from: 2020-12-14 Created: 2020-12-14 Last updated: 2023-12-11Bibliographically approved
Salvatori, R., Kehrein, K., Singh, A. P., Aftab, W., Vargas Möller-Hergt, B., Forne, I., . . . Ott, M. (2020). Molecular Wiring of a Mitochondrial Translational Feedback Loop. Molecular Cell, 77(4), 887-900
Open this publication in new window or tab >>Molecular Wiring of a Mitochondrial Translational Feedback Loop
Show others...
2020 (English)In: Molecular Cell, ISSN 1097-2765, E-ISSN 1097-4164, Vol. 77, no 4, p. 887-900Article in journal (Refereed) Published
Abstract [en]

The mitochondrial oxidative phosphorylation system comprises complexes assembled from subunits derived from mitochondrial and nuclear gene expression. Both genetic systems are coordinated by feedback loops, which control the synthesis of specific mitochondrial encoded subunits. Here, we studied how this occurs in the case of cytochrome b, a key subunit of mitochondrial complex III. Our data suggest the presence of a molecular rheostat consisting of two translational activators, Cbp3-Cbp6 and Cbs1, which operates at the mitoribosomal tunnel exit to connect translational output with assembly efficiency. When Cbp3-Cbp6 is engaged in assembly of cytochrome b, Cbs1 binds to the tunnel exit to sequester the cytochrome b-encoding mRNA, repressing its translation. After mediating complex III assembly, binding of Cbp3-Cbp6 to the tunnel exit replaces Cbs1 and the bound mRNA to permit cytochrome b synthesis. Collectively, the data indicate the molecular wiring of a feedback loop to regulate synthesis of a mitochondrial encoded protein.

National Category
Biochemistry Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:su:diva-159453 (URN)10.1016/j.molcel.2019.11.019 (DOI)000519769200017 ()31883951 (PubMedID)
Available from: 2018-08-29 Created: 2018-08-29 Last updated: 2025-02-20Bibliographically approved
Ndi, M., Marin-Buera, L., Salvatori, R., Singh, A. P. & Ott, M. (2018). Biogenesis of the bc(1) Complex of the Mitochondria! Respiratory Chain. Journal of Molecular Biology, 430(21), 3892-3905
Open this publication in new window or tab >>Biogenesis of the bc(1) Complex of the Mitochondria! Respiratory Chain
Show others...
2018 (English)In: Journal of Molecular Biology, ISSN 0022-2836, E-ISSN 1089-8638, Vol. 430, no 21, p. 3892-3905Article, review/survey (Refereed) Published
Abstract [en]

The oxidative phosphorylation system contains four respiratory chain complexes that connect the transport of electrons to oxygen with the establishment of an electrochemical gradient over the inner membrane for ATP synthesis. Due to the dual genetic source of the respiratory chain subunits, its assembly requires a tight coordination between nuclear and mitochondrial gene expression machineries. In addition, dedicated assembly factors support the step-by-step addition of catalytic and accessory subunits as well as the acquisition of redox cofactors. Studies in yeast have revealed the basic principles underlying the assembly pathways. In this review, we summarize work on the biogenesis of the bc(1) complex or complex III, a central component of the mitochondrial energy conversion system.

National Category
Biochemistry Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:su:diva-161925 (URN)10.1016/j.jmb.2018.04.036 (DOI)000448493400002 ()29733856 (PubMedID)
Available from: 2018-11-13 Created: 2018-11-13 Last updated: 2025-02-20Bibliographically approved
Singh, A. P., Salvatori, R., Aftab, W., Carlström, A., Forne, I., Imhof, A. & Ott, M.A protein proximity atlas reveals connectivity of mitochondrial translation and OXPHOS assembly at the ribosomal tunnel exit.
Open this publication in new window or tab >>A protein proximity atlas reveals connectivity of mitochondrial translation and OXPHOS assembly at the ribosomal tunnel exit
Show others...
(English)Manuscript (preprint) (Other academic)
National Category
Biochemistry Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:su:diva-180245 (URN)
Available from: 2020-03-24 Created: 2020-03-24 Last updated: 2025-02-20Bibliographically approved
Salvatori, R., Aftab, W., Imhof, A., Ott, M. & Singh, A. P.BioID as a tool to study protein-protein proximity in yeast mitochondria.
Open this publication in new window or tab >>BioID as a tool to study protein-protein proximity in yeast mitochondria
Show others...
(English)Manuscript (preprint) (Other academic)
National Category
Biochemistry Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:su:diva-180244 (URN)
Available from: 2020-03-24 Created: 2020-03-24 Last updated: 2025-02-20Bibliographically approved
Stephan, K., Ott, M. & Singh, A. The role of the assembly factor Bca1 and the structural subunit Qcr7 during bc1 complex biogenesis.
Open this publication in new window or tab >>The role of the assembly factor Bca1 and the structural subunit Qcr7 during bc1 complex biogenesis
(English)Manuscript (preprint) (Other academic)
Keywords
bc1 complex assembly, cytochrome b, complex III, oxidative phosphorylation, assembly factors
National Category
Biochemistry Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:su:diva-180248 (URN)
Available from: 2020-03-30 Created: 2020-03-30 Last updated: 2025-02-20Bibliographically approved
Organisations

Search in DiVA

Show all publications