Tigrinya is one of the Afro Semitic languages, traditionally classified as North Ethiopic, and spoken in Eritrean and Northern Ethiopia. In this work Tigrinya negation particles were investigated and analyzed. with the main aim to emphasise distribution of negation particles in different word classes in the language. It is designed to provide some analysis of how the North Afro Semitic languages are related, with some descriptions about how they have different distribution of negation forms. Some linguistic ideas in using Tigrinya negation by comparing it with its sister languages are also given.