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Oligomer Dynamics of LL-37 Truncated Fragments Probed by α-Hemolysin Pore and Molecular Simulations
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.ORCID iD: 0000-0003-0905-7911
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Number of Authors: 82023 (English)In: Small, ISSN 1613-6810, E-ISSN 1613-6829, Vol. 19, no 37, article id 2206232Article in journal (Refereed) Published
Abstract [en]

Oligomerization of antimicrobial peptides (AMPs) is critical in their effects on pathogens. LL-37 and its truncated fragments are widely investigated regarding their structures, antimicrobial activities, and application, such as developing new antibiotics. Due to the small size and weak intermolecular interactions of LL-37 fragments, it is still elusive to establish the relationship between oligomeric states and antimicrobial activities. Here, an α-hemolysin nanopore, mass spectrometry (MS), and molecular dynamic (MD) simulations are used to characterize the oligomeric states of two LL-37 fragments. Nanopore studies provide evidence of trapping events related to the oligomer formation and provide further details on their stabilities, which are confirmed by MS and MD simulations. Furthermore, simulation results reveal the molecular basis of oligomer dynamics and states of LL-37 fragments. This work provides unique insights into the relationship between the oligomer dynamics of AMPs and their antimicrobial activities at the single-molecule level. The study demonstrates how integrating methods allows deciphering single molecule level understanding from nanopore sensing approaches. 

Place, publisher, year, edition, pages
2023. Vol. 19, no 37, article id 2206232
Keywords [en]
dynamics, LL-37 fragments, nanopores, oligomers
National Category
Biophysics
Identifiers
URN: urn:nbn:se:su:diva-220214DOI: 10.1002/smll.202206232ISI: 000986112000001PubMedID: 37170734Scopus ID: 2-s2.0-85159128314OAI: oai:DiVA.org:su-220214DiVA, id: diva2:1791383
Available from: 2023-08-25 Created: 2023-08-25 Last updated: 2023-10-12Bibliographically approved

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Österlund, NicklasGräslund, Rut Astrid Olivia

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