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PepFects and NickFects for the Intracellular Delivery of Nucleic Acids
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi.ORCID-id: 0000-0001-8947-6643
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi. Tartu University, Estonia.
2015 (engelsk)Inngår i: Cell-Penetrating Peptides: Methods and Protocols / [ed] Ülo Langel, New York: Springer, 2015, Vol. 1324, s. 303-315Kapittel i bok, del av antologi (Fagfellevurdert)
Abstract [en]

Nucleic acids can be utilized in gene therapy to restore, alter, or silence gene functions. In order to reveal the biological activity nucleic acids have to reach their intracellular targets by passing through the plasma membrane, which is impermeable for these large and negatively charged molecules. Cell-penetrating peptides (CPPs) condense nucleic acids into nanoparticles using non-covalent complexation strategy and mediate their delivery into the cell, whereas the physicochemical parameters of the nanoparticles determine the interactions with the membranes, uptake mechanism, and subsequent intracellular fate. The nanoparticles are mostly internalized by endocytosis that leads to the entrapment of them in endosomal vesicles. Therefore design of new CPPs that are applicable for non-covalent complex formation strategy and harness endosomolytic properties is highly vital. Here we demonstrate that PepFects and NickFects are efficient vectors for the intracellular delivery of various nucleic acids.This chapter describes how to form CPP/pDNA nanoparticles, evaluate stable nanoparticles formation, and assess gene delivery efficacy.

sted, utgiver, år, opplag, sider
New York: Springer, 2015. Vol. 1324, s. 303-315
Serie
Methods in Molecular Biology, ISSN 1064-3745 ; 1324
Emneord [en]
Cell-penetrating peptide, Co-incubation, Nanoparticle formation, Oligonucleotide delivery, PepFect, NickFect
HSV kategori
Identifikatorer
URN: urn:nbn:se:su:diva-124907DOI: 10.1007/978-1-4939-2806-4_19ISI: 000376037700020PubMedID: 26202277ISBN: 978-1-4939-2805-7 (tryckt)ISBN: 978-1-4939-2806-4 (tryckt)OAI: oai:DiVA.org:su-124907DiVA, id: diva2:891239
Tilgjengelig fra: 2016-01-06 Laget: 2016-01-06 Sist oppdatert: 2020-03-05bibliografisk kontrollert

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