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Rosiglitazone and a beta(3)-Adrenoceptor Agonist Are Both Required for Functional Browning of White Adipocytes in Culture
Vise andre og tillknytning
Rekke forfattare: 102018 (engelsk)Inngår i: Frontiers in Endocrinology, ISSN 1664-2392, E-ISSN 1664-2392, Vol. 9, artikkel-id 249Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The recruitment of brite (or beige) adipocytes has been advocated as a means to combat obesity, due to their ability to phenotypically resemble brown adipocytes (BA). Lineage studies indicate that brite adipocytes are formed by differentiation of precursor cells or by direct conversion of existing white adipocytes, depending on the adipose depot examined. We have systematically compared the gene expression profile and a functional output (oxygen consumption) in mouse adipocytes cultured from two contrasting depots, namely interscapular brown adipose tissue, and inguinal white adipose tissue (iWAT), following treatment with a known browning agent, the peroxisome proliferator-activated receptor (PPAR gamma) activator rosiglitazone. Prototypical BA readily express uncoupling protein (UCP)1, and upstream regulators including the beta(3)-adrenoceptor and transcription factors involved in energy homeostasis. Adipocytes from inguinal WAT display maximal UCP1 expression and mitochondrial uncoupling only when treated with a combination of the PPAR. activator rosiglitazone and a beta(3)-adrenoceptor agonist. In conclusion, brite adipocytes are fully activated only when a browning agent (rosiglitazone) and a thermogenic agent (beta(3)-adrenoceptor agonist) are added in combination. The presence of rosiglitazone throughout the 7-day culture period partially masks the effects of beta(3)-adrenoceptor signaling in inguinal white adipocyte cultures, whereas including rosiglitazone only for the first 3 days promotes robust beta(3)-adrenoceptor expression and provides an improved window for detection of beta(3)-adrenoceptor responses.

sted, utgiver, år, opplag, sider
2018. Vol. 9, artikkel-id 249
Emneord [en]
adipocyte, beta adrenergic receptors, uncoupling protein 1, adrenoceptor, seahorse xf96 analysis, rosiglitazone, CL316243
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Identifikatorer
URN: urn:nbn:se:su:diva-157718DOI: 10.3389/fendo.2018.00249ISI: 000433550500001PubMedID: 29910772OAI: oai:DiVA.org:su-157718DiVA, id: diva2:1236273
Tilgjengelig fra: 2018-08-01 Laget: 2018-08-01 Sist oppdatert: 2018-08-01bibliografisk kontrollert

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