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Microfluidic electrocapture-assisted mass spectrometry of membrane-associated polypeptides
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.ORCID-id: 0000-0003-3678-7100
2008 (engelsk)Inngår i: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 80, s. 7116-7120Artikkel i tidsskrift (Fagfellevurdert) Published
sted, utgiver, år, opplag, sider
ACS , 2008. Vol. 80, s. 7116-7120
HSV kategori
Identifikatorer
URN: urn:nbn:se:su:diva-17511ISI: 000259199400035OAI: oai:DiVA.org:su-17511DiVA, id: diva2:184032
Tilgjengelig fra: 2009-01-16 Laget: 2009-01-16 Sist oppdatert: 2022-02-25bibliografisk kontrollert
Inngår i avhandling
1. Novel clean-up, concentration and laser desorption/ionization strategies for mass spectrometry
Åpne denne publikasjonen i ny fane eller vindu >>Novel clean-up, concentration and laser desorption/ionization strategies for mass spectrometry
2010 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Despite valuable advantages of mass spectrometry (MS) techniques they still have drawbacks that need to be overcome, notably their sensitivity to various kinds of interfering substances and associated difficulties involved in detecting signals from analytes present in trace quantities. Thus, high quality analytical data could be acquired by improving sample pre-treatment techniques and/or ionization methods to increase ionization yields and/or avoid the generation of unwanted ions.

In the studies this thesis is based upon, a microfluidic electrocapture system capable of trapping charged proteins/polypeptides while non-charged species such as non-ionic detergents are swept out, was used to decrease the concentration of detergent in samples containing soluble and membrane associated proteins/polypeptide. Subsequent mass spectrometric analysis yielded well-resolved mass spectra of the target analytes.

In addition, it was found that solid phase extraction (SPE) sorbents such as graphitized carbon black (GCB), can efficiently assist the laser desorption ionization of small molecules without generating any interfering ions which inspired the fabrication of μ-traps and discs. Other matrix-free approaches demonstrated the utility of silicon nitride and oxidized GCB nanoparticles as versatile media for surface-assisted laser desorption ionization (SALDI) of small molecules.

In this thesis it is discussed that small molecule analytes such as Polyphenols present in red wine and quaternary protoberberine alkaloids with highly conjugated systems and acidic/permanently charged functional groups can be readily analyzed without any matrix or assisting-surface.

In summary, the microfluidic electrocapture, new laser desorption ionization methods and surfaces, as well as integrated SALDI and SPE platforms, introduced in this work extend the MS strategies allowing coverage of a wider range of samples.

sted, utgiver, år, opplag, sider
Stockholm: Department of Analytical Chemistry, Stockholm University, 2010. s. 77
Emneord
Mass spectrometry, ESI-MS, SALDI-MS, Electrocapture, Sample treatment
HSV kategori
Forskningsprogram
analytisk kemi
Identifikatorer
urn:nbn:se:su:diva-32236 (URN)978-91-7155-981-4 (ISBN)
Disputas
2010-01-22, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 10:00 (engelsk)
Opponent
Veileder
Merknad
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: In PressTilgjengelig fra: 2009-12-17 Laget: 2009-12-07 Sist oppdatert: 2022-02-25bibliografisk kontrollert

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Shariatgorji, MohammadrezaIlag, Leopold

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