Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Structural and conformational studies of bacterial polysaccharides employing NMR spectroscopy
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för organisk kemi.
2000 (engelsk)Doktoravhandling, monografi (Annet vitenskapelig)
Abstract [en]

Methods and approaches for performing structural and conformational analysis of bacterial polysaccharides in solution are described. The structure of one exopolysaccharide from Lactobacillus helveticus and two O-antigens from Escherichia coli have been determined using NMR spectroscopy, chemical degradation and mass spectrometry. This thesis discusses different problems encountered in connection with these structural elucidations, namely, overlap of NMR signals, heterogeneity in the polysaccharide sequence and the configurations of a nonulosonic acid.

A conformational analysis of the enterobacterial common antigen (ECA) in the form of a cyclic dodecamer is described. Nuclear Overhauser effects (NOEs) and heteronuclear three-bond coupling constants (3JCH) over the glycosidic linkages were determined and used to calculate proton-proton distances and torsion angles. Residual dipolar couplings were measured in a liquid crystalline medium. These couplings provide orientational information distinct from that contained in the local NOE and 3JCH data. The experimental data were compared to the averaged distances, three-bond proton-carbon coupling constants and C-H vector orientations in a model obtained by molecular dynamics simulations. From a fragment that were in agreement with experimental data, a three-dimensional structure was generated for the cyclic ECA in solution

sted, utgiver, år, opplag, sider
Stockholm: Department of Organic Chemistry, Stockholm University , 2000. , s. 42
Emneord [en]
Bacterial polysaccharides, Magnetic resonance spectroscopy
HSV kategori
Forskningsprogram
organisk kemi
Identifikatorer
URN: urn:nbn:se:su:diva-33021Libris ID: 7629874ISBN: 91-7265-068-0 (tryckt)OAI: oai:DiVA.org:su-33021DiVA, id: diva2:282202
Disputas
2000-04-28, Magnélisalen, KÖL, Stockholm, 10:00 (engelsk)
Opponent
Merknad

Härtill 4 uppsatser

Tilgjengelig fra: 2009-12-18 Laget: 2009-12-18 Sist oppdatert: 2019-01-08bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

PDF (Not accessible to users outside Sweden)
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric

isbn
urn-nbn
Totalt: 105 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf