Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Electron-Capture-Induced Dissociation of Microsolvated Di- and > Tripeptide Monocations: Elucidation of Fragmentation Channels from > Measurements of Negative Ions
Stockholms universitet, Naturvetenskapliga fakulteten, Fysikum. (Atomfysik)
Stockholms universitet, Naturvetenskapliga fakulteten, Fysikum. (Atomfysik)
Vise andre og tillknytning
2009 (engelsk)Inngår i: ChemPhysChem, ISSN 1439-4235, E-ISSN 1439-7641, Vol. 10, nr 9-10, s. 1619-1623Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The branching ratio between ammonia loss and NCα bond cleavage of singly charged microsolvated peptides after electron capture from cesium depends on the solvent molecule attached. Density functional calculations reveal that for [GA+H]+(CE) (G=glycine, A=alanine, CE=crown ether), the singly occupied molecular orbital of the neutral radical is located mainly on the amide group (see picture).

The results from an experimental study of bare and microsolvated peptide monocations in high-energy collisions with cesium vapor are reported. Neutral radicals form after electron capture from cesium, which decay by H loss, NH3 loss, or NCα bond cleavage into characteristic z. and c fragments. The neutral fragments are converted into negatively charged species in a second collision with cesium and are identified by means of mass spectrometry. For protonated GA (G=glycine, A=alanine), the branching ratio between NH3 loss and NCα bond cleavage is found to strongly depend on the molecule attached (H2O, CH3CN, CH3OH, and 18-crown-6 ether (CE)). Addition of H2O and CH3OH increases this ratio whereas CH3CN and CE decrease it. For protonated AAA ([AAA+H]+), a similar effect is observed with methanol, while the ratio between the z1 and z2 fragment peaks remains unchanged for the bare and microsolvated species. Density functional theory calculations reveal that in the case of [GA+H]+(CE), the singly occupied molecular orbital is located mainly on the amide group in accordance with the experimental results.

sted, utgiver, år, opplag, sider
2009. Vol. 10, nr 9-10, s. 1619-1623
Emneord [en]
cations, electron transfer, excited states, mass spectrometry, peptides
HSV kategori
Forskningsprogram
fysik
Identifikatorer
URN: urn:nbn:se:su:diva-33290DOI: 10.1002/cphc.200800782ISI: 000267928100039OAI: oai:DiVA.org:su-33290DiVA, id: diva2:282900
Tilgjengelig fra: 2009-12-22 Laget: 2009-12-22 Sist oppdatert: 2017-12-12bibliografisk kontrollert
Inngår i avhandling
1. Fragmentation of Amino Acids and Microsolvated Peptides and Nucleotides using Electrospray Ionization Tandem Mass Spectrometry
Åpne denne publikasjonen i ny fane eller vindu >>Fragmentation of Amino Acids and Microsolvated Peptides and Nucleotides using Electrospray Ionization Tandem Mass Spectrometry
2010 (engelsk)Licentiatavhandling, med artikler (Annet vitenskapelig)
Abstract [en]

This thesis presents three different series of high energy (keV) collision experiments as well as a brief scientific introduction to the field. In the first series, protonated glycine and leucine were collided with carbon dioxide and a beam attenuation method was applied to determine their total fragmentation cross sections. A technique was also presented for how to restore the resolution in mass spectra obtained with a hemispherical electrostatic analyzer followed by a position sensitive detector (micro-channel plate equipped with a resistive anode). In the second series of experiments, Collision Induced Dissociation (CID) and Electron Capture Induced Dissociation (ECID) studies were performed on the nucleotide adenosine 5'-monophosphate anion (AMP-) in water complexes.  The two dissociation techniques revealed different fragmentation patterns and a numerical solvent evaporation model was used to interpret the spectra. It was then found that the CID and ECID processes were associated with different internal energy distributions. The third experiment concerned ECID of the protonated dipeptide glycine-alanine ([GA+H]+) in complexes with water, methanol, acetonitrile or crown ether. Depending on the attached molecular species, different ratios between the two competing channels ammonia loss and N-Cα bond cleavage were observed. Quantum chemical calculations revealed that a notable shift in the location of the captured electron occurred for the case of two acetonitriles and one crown ether compared to the bare ion and the ion in complexes with either water or methanol. Finally, this thesis will discuss developments of the electrospray ionization platform as well as the new Double ElectroStatic IonRing ExpEriment (DESIREE) facility.

Publisher
s. 80
HSV kategori
Forskningsprogram
fysik
Identifikatorer
urn:nbn:se:su:diva-37202 (URN)
Presentation
2010-02-15, FA31, Albanova universitetscentrum, Roslagstullsbacken 21, Stockholm, 13:15 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2011-02-18 Laget: 2010-02-17 Sist oppdatert: 2011-02-18bibliografisk kontrollert
2. Probing biomolecular fragmentation
Åpne denne publikasjonen i ny fane eller vindu >>Probing biomolecular fragmentation
2011 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

This thesis deals with fragmentation of complex molecular ions, especially biomolecules, in gas phase collision experiments. The aim is to investigate the relations between energy deposition and fragmentation and to shed light on the mechanisms behind energy and charge transfer processes in collisions involving the building blocks of life. Further, the question how a solvent environment influences the dissociation behavior is elucidated. In the first part of the thesis, results from different collision experiments with biomolecular ions are presented, focusing on electron capture induced dissociation of hydrated nucleotides and small peptides. The investigated processes may be relevant for the understanding of radiation damage and the optimization of sequencing methods used in protein research. Our results clearly demonstrate that effects due to surrounding solvent molecules are substantial. While the dissipation of internal energy by evaporation of the loosely bound solvent molecules may protect the biomolecule, the influence which this environment has on the electronic structure may lead to an enhancement or suppression of certain dissociation channels. The second part of the thesis focuses on recent instrumental developments. Here, the aim was to optimize and complement the techniques used in the experiments above and to have versatile tools available for different kinds of gas phase collision studies involving complex molecular ions. Therefore, we have constructed an electrospray ion source platform for the preparation of intense beams, with options of accumulation and cooling of mass selected ions, allowing for a large variety of experiments. This device is also intended to serve as an ion source for the new storage ring facility DESIREE (DoubleElectroStatic Ion Ring ExpEriment), which is currently under construction at Stockholm University. In these unique storage rings, oppositely charged ions may interact at very low relative velocities in a cryogenically cooled and ultrahigh vacuum environment.

sted, utgiver, år, opplag, sider
Stockholm: Department of Physics, Stockholm University, 2011. s. 124
HSV kategori
Forskningsprogram
fysik
Identifikatorer
urn:nbn:se:su:diva-54524 (URN)978-91-7447-228-8 (ISBN)
Disputas
2011-03-11, FB55, AlbaNova universitetscentrum, Roslagstullsbacken 21, Stockholm, 13:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2011-02-17 Laget: 2011-02-04 Sist oppdatert: 2011-02-07bibliografisk kontrollert
3. Ionization and Fragmentation of Complex Molecules and Clusters: Biomolecules and Polycyclic Aromatic Hydrocarbons
Åpne denne publikasjonen i ny fane eller vindu >>Ionization and Fragmentation of Complex Molecules and Clusters: Biomolecules and Polycyclic Aromatic Hydrocarbons
2011 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

This work deals with ionization and fragmentation of biomolecules and polycyclic aromatic hydrocarbon (PAH) molecules. They are studied in the gas phase both as isolated molecules and as weakly bound clusters. The purpose of the experimental and theoretical investigations are to elucidate charge and energy transfer and related redistribution processes, as well as fragmentation behaviors.

The first part of this thesis presents results from studies on biomolecular ions, in particular nucleotides and peptides, which are primarily examined in electron capture induced dissociation processes. These investigations are relevant for the better understanding of radiation damage to DNA and processes involved in the sequencing of proteins. It is found that the immediate environment have a decisive influence on the fragmentation behaviors. Evaporation of surrounding molecules protect the biomolecules, but their effect on the electronic structure may also enhance or suppress different fragmentation channels.

In the second part of the thesis, results from studies on PAH molecules are presented. Experimentally, their properties are mainly probed through collisions with atomic ion projectiles having kilo-electronvolt kinetic energies. As a widespread pollutant on Earth, and as a family of abundant molecules in space, PAHs are not only relevant from an environmental and health perspective, but they are also important for the understanding of the universe. The present results relate to the stabilities of these molecules, both in isolated form and in clusters, when heated or multiply ionized. It is found to be easier to remove several electrons from clusters of PAH molecules than from isolated PAHs, and fission processes determine their ultimate stabilities. Heated low-charge state clusters of PAHs undergo long evaporation sequences once these have started. For isolated and heated PAHs, internal structural rearrangements are demonstrated to be important in the fragmentation processes.

sted, utgiver, år, opplag, sider
Stockholm: Department of Physics, Stockholm University, 2011. s. 168
HSV kategori
Forskningsprogram
fysik
Identifikatorer
urn:nbn:se:su:diva-63733 (URN)978-91-7447-399-5 (ISBN)
Disputas
2011-12-02, lecture room FB53, AlbaNova universitetscentrum, Roslagstullsbacken 21, Stockholm, 13:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2011-11-10 Laget: 2011-10-27 Sist oppdatert: 2011-11-01bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekst

Søk i DiVA

Av forfatter/redaktør
Zettergren, HenningCederquist, HenrikHaag, NicoleHolm, Anne I. S.Johansson, Henrik A. B.Reinhed, PeterSchmidt, Henning T.
Av organisasjonen
I samme tidsskrift
ChemPhysChem

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 205 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf