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SNARE-fusion mediated insertion of membrane proteins into native and artificial membranes
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik. University of Bern, Switzerland.
2014 (engelsk)Inngår i: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 5, s. 4303-Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Membrane proteins carry out functions such as nutrient uptake, ATP synthesis or transmembrane signal transduction. An increasing number of reports indicate that cellular processes are underpinned by regulated interactions between these proteins. Consequently, functional studies of these networks at a molecular level require co-reconstitution of the interacting components. Here, we report a SNARE protein-based method for incorporation of multiple membrane proteins into artificial membrane vesicles of well-defined composition, and for delivery of large water-soluble substrates into these vesicles. The approach is used for in vitro reconstruction of a fully functional bacterial respiratory chain from purified components. Furthermore, the method is used for functional incorporation of the entire F1F0 ATP synthase complex into native bacterial membranes from which this component had been genetically removed. The novel methodology offers a tool to investigate complex interaction networks between membrane-bound proteins at a molecular level, which is expected to generate functional insights into key cellular functions.

sted, utgiver, år, opplag, sider
2014. Vol. 5, s. 4303-
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URN: urn:nbn:se:su:diva-107442DOI: 10.1038/ncomms5303ISI: 000340613800011OAI: oai:DiVA.org:su-107442DiVA, id: diva2:747601
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AuthorCount:3;

Tilgjengelig fra: 2014-09-17 Laget: 2014-09-15 Sist oppdatert: 2017-12-05bibliografisk kontrollert

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