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CD4(+) T cell activation, function, and metabolism are inhibited by low concentrations of DMSO
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Antal upphovsmän: 72018 (Engelska)Ingår i: JIM - Journal of Immunological Methods, ISSN 0022-1759, E-ISSN 1872-7905, Vol. 463, s. 54-60Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Dimethyl sulfoxide (DMSO) is a polar organic solvent used in a wide range of biological applications. DMSO is routinely used as a cryoprotectant for long-term cell freezing as well as to dissolve peptides and drugs for immune cell functional assays. Here, human CD4(+) T cell activation, cytokine production, proliferation, and metabolism were investigated after stimulation in the presence of 0.01% to 1%, DMSO, representing concentrations commonly used in vitro. Surface expression of the activation markers CD69, CD25 and CD154 after polyclonal activation of CD4(+) T cells was inhibited by 0.25% or higher concentrations of DMSO. The frequencies of IL-21(+), IL-4(+), and IL-22(+) CD4(+) T cells, following polyclonal activation were variably inhibited by DMSO at concentrations ranging from 0.25% to 1%, whereas IFN gamma(+) cells were unaffected. CD4(+) T cell proliferation after anti-CD3 or antigen stimulation was inhibited by 0.5% DMSO and abolished by 1% DMSO. After polyclonal stimulation, glucose uptake was inhibited in the presence of 1% DMSO, but only minor effects on CD4+ T cell respiration were observed. Consistent with the immune effects, the gene expression of early signaling and activation pathways were inhibited in CD4+ T cells in the presence of 1% DMSO. Our study revealed that DMSO at concentrations generally used for in vitro studies of T cells impacts multiple features of T cell function. Therefore, we urge care when adding DMSO-containing preparations to T cell cultures.

Ort, förlag, år, upplaga, sidor
2018. Vol. 463, s. 54-60
Nationell ämneskategori
Biologiska vetenskaper Cell- och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:su:diva-163564DOI: 10.1016/j.jim.2018.09.004ISI: 000453496300006PubMedID: 30201392OAI: oai:DiVA.org:su-163564DiVA, id: diva2:1279188
Tillgänglig från: 2019-01-16 Skapad: 2019-01-16 Senast uppdaterad: 2019-01-16Bibliografiskt granskad

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Av författaren/redaktören
Jastroch, Martin
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Institutionen för molekylär biovetenskap, Wenner-Grens institut
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JIM - Journal of Immunological Methods
Biologiska vetenskaperCell- och molekylärbiologi

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