For dendroclimatological Blue Intensity (BI) studies based on earlywood (EW) or latewood (LW) information, a demarcation between the two is necessary, which can be difficult to establish for species where the transition is subtle. Often, a percental value k is used that calculates an EW/LW boundary value for each tree ring individually based on the difference between maximum and minimum absorption. Several laboratories and authors have used different values for k (e.g. k = 30 % or k = 50 %), while wood anatomical and visual studies suggest that k is on the order of 80 %. Here, we test how different settings of k, and thus different definitions of the EW and LW proportions of a tree ring, influence the dendroclimatic potential of derived time series. To this end, we correlate instrumental temperature measurements with tree ring chronologies that are based on EW and LW information (e.g. EW absorption (EWBI), LW absorption (LWBI)), where the EW/LW proportion is varied by setting different values for k. The tree ring samples utilized are 30 cores of spruce (Picea abies) trees from a high-elevated site (ca. 1700 m a.s.l.) in the northern Alps, Austria. Overall, we achieve high correlations between temperature data and our tree ring chronologies. Regarding the stability of the climate signal under different k values, the results show that absorption intensity based parameters (Delta BI, EWBI, LWBI) are only mildly influenced by different settings of k, while width based parameters (EW width, LW width) show a larger dependence on k. LW width, for instance, was stronger correlated with temperature, the smaller the LW was chosen (and thus the higher k was set). Based on our results and the wood anatomical definition of the EW/LW boundary, we suggest that k = 80 % may be a good choice for future studies. However, since this is only a case study from one site, careful screening of the respective data set regarding an appropriate k value must accompany each dendroclimatological study.