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Analytical protocol for identification of BMAA and DAB in biologicalsamples
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.
Stockholms universitet, Naturvetenskapliga fakulteten, Botaniska institutionen.
Stockholms universitet, Naturvetenskapliga fakulteten, Botaniska institutionen.
Stockholms universitet, Naturvetenskapliga fakulteten, Botaniska institutionen.
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2010 (Engelska)Ingår i: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 135, s. 127-132Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

 

b

-N-methylamino-L-alanine (BMAA) is a non-protein amino acid, thought to be inflicting neurodegenerative diseases related to ALS/PDC in human beings. Due to conflicting data concerning the presence of BMAA in various biological matrixes, we present a robust and sensitive method for high confidence identification of BMAA after derivatization by 6-aminoquinolyl-N

-hydroxysuccinimidyl carbamate (AQC). The efficient sample pretreatment in combination with LC-MS/MS SRM enables chromatographic separation of BMAA from the isomer 2,3-diaminobutyric acid (DAB). The method is applicable for selective BMAA/DAB detection in various biological samples ranging from a prokaryotic cyanobacterium to eukaryotic fish.

Ort, förlag, år, upplaga, sidor
The Royal Society of Chemistry , 2010. Vol. 135, s. 127-132
Nationell ämneskategori
Biologiska vetenskaper
Identifikatorer
URN: urn:nbn:se:su:diva-33260DOI: 10.1039/b921048bOAI: oai:DiVA.org:su-33260DiVA, id: diva2:282781
Tillgänglig från: 2009-12-21 Skapad: 2009-12-21 Senast uppdaterad: 2020-02-06Bibliografiskt granskad
Ingår i avhandling
1. Mass Spectrometry of Biologically Active Small Molecules: Focusing on polyphenols, alkaloids and amino acids
Öppna denna publikation i ny flik eller fönster >>Mass Spectrometry of Biologically Active Small Molecules: Focusing on polyphenols, alkaloids and amino acids
2010 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

The foci of this dissertation are on advanced liquid chromatography (LC) separation and mass spectrometry (MS) techniques for the analysis of small bioactive molecules. In addition to discussing general aspects of such techniques the results from analyses of polyphenols (PPs), alkaloids and amino acids published in five appended studies are presented and discussed. High efficiency and well understood principles make LC the method of choice for separating analytes in many kinds of scientific investigations. Moreover, when LC is coupled to an MS instrument, analytes are separated in two stages: firstly they are separated and pre-concentrated in narrow bands using LC and then separated according to their mass-to-charge (m/z) ratios in the MS instrument. Some MS instruments can provide highly accurate molecular weight measurements and mass resolution allowing identification of unknown compounds based purely on MS data, thus making prior separation unnecessary. However, prior separation is essential for analyzing substances in most complex matrices – especially useful is the ultra-high performance LC (UHPLC). The advantages of using UHPLC rather than HPLC for the analysis of PPs in tea and wine were evaluated in one of the studies this thesis is based upon. The phenolic composition of red wine was also examined, using a novel LDI technique, following solid phase extraction (SPE). A class of small aromatic molecules (medicinally important alkaloids) also proved to be amenable to straightforward analysis, by thin layer chromatography (TLC) work-up followed by LDI-MS. Finally, a LC-MS method for monitoring neurotoxins (β-N-methyl-amino-L-alanine and 2,3-diaminobutyric acid) in complex biological matrices was developed and applied. Overall, the studies show that careful attention to the physicochemical properties of analytes can provide insights that can greatly facilitate the development of alternative methods to analyze them, e.g. by LDI.

Ort, förlag, år, upplaga, sidor
Stockholm: Department of Analytic Chemistry, Stockholm University, 2010. s. 61
Nyckelord
LC-MS, LDI, Mass spectrometry, HPLC, UHPLC, polyphenols, phenolic acids, BMAA
Nationell ämneskategori
Analytisk kemi Analytisk kemi
Forskningsämne
analytisk kemi
Identifikatorer
urn:nbn:se:su:diva-33233 (URN)978-91-7155-982-1 (ISBN)
Disputation
2010-01-29, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 13:00 (Engelska)
Opponent
Handledare
Anmärkning

At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 4: In press. Paper 5: Manuscript.

Tillgänglig från: 2010-01-07 Skapad: 2009-12-21 Senast uppdaterad: 2015-06-08Bibliografiskt granskad

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Spacil, ZdenekEriksson, JohanJonasson, SaraRasmussen, UllaIlag, Leopold L.Bergman, Birgitta
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Institutionen för analytisk kemiBotaniska institutionen
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The Analyst
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