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Contrasting effects of cold acclimation versus obesogenic diets onchemerin gene expression in brown and brite adipose tissues
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för molekylär biovetenskap, Wenner-Grens institut.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för molekylär biovetenskap, Wenner-Grens institut.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för molekylär biovetenskap, Wenner-Grens institut.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för molekylär biovetenskap, Wenner-Grens institut.
(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Abstract [en]

Based on results from a signal sequence trap, we investigated chemerin gene expression in brown adipose tissue. Male NMRI mice were exposed to 30, 22 or 4 °C for 3 weeks, or were fed control (chow) diet, cafeteria diet or high-fat diet at thermoneutrality for the same time. In brown adipose tissue, cold acclimation strongly diminished chemerin gene expression, whereas obesogenic diets augmented expression. Qualitatively, changes in expression were paralleled in brite/beige adipose tissues (e.g. inguinal), whereas white adipose tissue (epididymal) did not react to these cues. Changes in tissue expression were not paralleled by alterations in plasma levels. The cellular regulation was not congruent with a sympathetic/adrenergic control of expression. The data are discussed in relation to suggested endocrine, paracrine and autocrine effects of this adipokine.

Nationell ämneskategori
Naturvetenskap Biokemi och molekylärbiologi
Forskningsämne
fysiologi
Identifikatorer
URN: urn:nbn:se:su:diva-102923OAI: oai:DiVA.org:su-102923DiVA, id: diva2:714060
Tillgänglig från: 2014-04-25 Skapad: 2014-04-25 Senast uppdaterad: 2014-04-25
Ingår i avhandling
1. The secretome of brown adipose tissue
Öppna denna publikation i ny flik eller fönster >>The secretome of brown adipose tissue
2014 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Brown adipose tissue has long been known for its heat-producing capacity, but less is known about its possible effects as a secretory organ. This thesis summarizes information about presently known factors secreted from brown adipose tissue and about their actions. We were able to add factors to the list by the use of a signal-sequence trap method. Results from the signal-sequence trap generated a list of suggested brown adipocyte secreted proteins; gene expression of these proteins was then further studied with microarray technique.

One of the genes further analyzed was the adipokine chemerin. Gene expression of chemerin in brown adipose tissue was decreased in cold acclimation but increased with a high-caloric diet. This indicates that factors other than norepinephrine influence chemerin gene expression. The effects on chemerin gene expression were not be reflected in serum levels; therefore, chemerin secreted from brown adipose tissue is ascribed an autocrine/paracrine role.

Signal-sequence trap and microarray studies suggested adrenomedullin, collagen type 3 a1, lipocalin 2 and Niemann Pick type C2 to be highly secreted from brown adipocytes. Gene expression of these factors was examined in vivo and in vitro. Our studies showed that both cold acclimation and high-caloric diet have an effect on gene expression of these factors. However, there was no effect on gene expression of chemerin and collagen type 3 a1 in norepinephrine-treated brown adipocyte cell cultures. This suggests that effects on gene expression of the examined possible brown adipocyte secreted proteins are not solely controlled by norepinephrine.

Ort, förlag, år, upplaga, sidor
Stockholm: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 2014. s. 101
Nationell ämneskategori
Biokemi och molekylärbiologi
Forskningsämne
fysiologi
Identifikatorer
urn:nbn:se:su:diva-102934 (URN)978-91-7447-903-4 (ISBN)
Disputation
2014-05-28, Lecture hall E306, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 10:00 (Engelska)
Opponent
Handledare
Anmärkning

At the time of doctoral defence the following papers were unpublished and had a status as follows: Paper 1: Manuscript; Paper 3: Manuscript; Paper  4: Manuscript; Paper 5: Manuscript

Tillgänglig från: 2014-05-06 Skapad: 2014-04-25 Senast uppdaterad: 2014-04-28Bibliografiskt granskad

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Av författaren/redaktören
Hansen, IdaCannon, BarbaraNedergaard, Jan
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Institutionen för molekylär biovetenskap, Wenner-Grens institut
NaturvetenskapBiokemi och molekylärbiologi

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