Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Effects of differentiation on gene expression of certain brown adipocyte-secreted factors
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för molekylär biovetenskap, Wenner-Grens institut.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för molekylär biovetenskap, Wenner-Grens institut.
(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Abstract [en]

The possibility that brown adipose tissue can secrete factors to affect overall metabolism is a subject that attracts attention. Previous studies from our laboratory suggest that chemerin and collagen type III a1 (Col3a1) could be interesting factors secreted from brown adipose tissue.

To evaluate chemerin and Col3a1 gene expression regulation, primary brown adipocyte cultures were stimulated with norepinephrine at different stages of differentiation. The present study shows that there was an effect of differentiation on gene expression of these factors but there was no effect of norepinephrine treatment.

There has been a suggestion that different adenylate cyclases are responsible for regulating gene expression in brown adipocytes. However, neither differentiation nor norepinephrine treatment affected adenylate cyclase gene expression dramatically.

The results suggest that norepinephrine is not the main regulator of gene expression of either of the two possible brown adipocyte-secreted factors, chemerin and Col3a1. Surprisingly, norepinephrine had no major effect on adenylate cyclase expression at any stage of differentiation.

Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:su:diva-102933OAI: oai:DiVA.org:su-102933DiVA, id: diva2:714070
Tillgänglig från: 2014-04-25 Skapad: 2014-04-25 Senast uppdaterad: 2014-04-25
Ingår i avhandling
1. The secretome of brown adipose tissue
Öppna denna publikation i ny flik eller fönster >>The secretome of brown adipose tissue
2014 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Brown adipose tissue has long been known for its heat-producing capacity, but less is known about its possible effects as a secretory organ. This thesis summarizes information about presently known factors secreted from brown adipose tissue and about their actions. We were able to add factors to the list by the use of a signal-sequence trap method. Results from the signal-sequence trap generated a list of suggested brown adipocyte secreted proteins; gene expression of these proteins was then further studied with microarray technique.

One of the genes further analyzed was the adipokine chemerin. Gene expression of chemerin in brown adipose tissue was decreased in cold acclimation but increased with a high-caloric diet. This indicates that factors other than norepinephrine influence chemerin gene expression. The effects on chemerin gene expression were not be reflected in serum levels; therefore, chemerin secreted from brown adipose tissue is ascribed an autocrine/paracrine role.

Signal-sequence trap and microarray studies suggested adrenomedullin, collagen type 3 a1, lipocalin 2 and Niemann Pick type C2 to be highly secreted from brown adipocytes. Gene expression of these factors was examined in vivo and in vitro. Our studies showed that both cold acclimation and high-caloric diet have an effect on gene expression of these factors. However, there was no effect on gene expression of chemerin and collagen type 3 a1 in norepinephrine-treated brown adipocyte cell cultures. This suggests that effects on gene expression of the examined possible brown adipocyte secreted proteins are not solely controlled by norepinephrine.

Ort, förlag, år, upplaga, sidor
Stockholm: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, 2014. s. 101
Nationell ämneskategori
Biokemi och molekylärbiologi
Forskningsämne
fysiologi
Identifikatorer
urn:nbn:se:su:diva-102934 (URN)978-91-7447-903-4 (ISBN)
Disputation
2014-05-28, Lecture hall E306, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 10:00 (Engelska)
Opponent
Handledare
Anmärkning

At the time of doctoral defence the following papers were unpublished and had a status as follows: Paper 1: Manuscript; Paper 3: Manuscript; Paper  4: Manuscript; Paper 5: Manuscript

Tillgänglig från: 2014-05-06 Skapad: 2014-04-25 Senast uppdaterad: 2014-04-28Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Sök vidare i DiVA

Av författaren/redaktören
Hansen, IdaCannon, Barbara
Av organisationen
Institutionen för molekylär biovetenskap, Wenner-Grens institut
Biokemi och molekylärbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 76 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf