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The structures of three bacterial polysaccharides and model studies on oligosaccharides and polyisoprenoids using NMR and FAB-MS
Stockholm University, Faculty of Science, Department of Organic Chemistry.
1993 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Some vicinally branched milk and blood group oligosaccharides were analyzed using nuclear magnetic resonance (NMR) spectroscopy in order to investigate NMR chemical shift changes that originate from the branching. These 13C-NMR chemical shift changes were used to calculate NMR spectra of oligo- and polysaccharides using the computer program CASPER.

The structures of three bacterial polysaccharides from Aerococcus viridans var. homari, Fusobacterium necrophorum and Vibrio cholerae 0:5 were investigated using NMR spectroscopy, fast atom bombardment mass spectrometry (FAB-MS) and chemical degradation as the principal methods. All of these polysaccharides contained unusual sugars and non-sugar components, some of which had not been found in nature previously.

FAB-MS was used for determination of structural features of polyisoprenoids. Different matrices and additives for positive ion FAB-MS of model polyprenol and dolichol compounds were investigated. Negative ion FAB-MS spectra showed peaks for the molecular ions of dolichyl phosphates from rat liver extracts.

Place, publisher, year, edition, pages
Stockholm: Stockholm University, 1993. , p. 52
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:su:diva-153395ISBN: 91-7153-087-8 (print)OAI: oai:DiVA.org:su-153395DiVA, id: diva2:1186093
Note

Härtill 5 uppsatser

Available from: 2018-02-27 Created: 2018-02-27 Last updated: 2018-02-27Bibliographically approved

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