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Characterization of the quinol-dependent nitric oxide reductase from the pathogen Neisseria meningitidis, an electrogenic enzyme
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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Number of Authors: 112018 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 8, article id 3637Article in journal (Refereed) Published
Abstract [en]

Bacterial nitric oxide reductases (NORs) catalyse the reduction of NO to N2O and H2O. NORs are found either in denitrification chains, or in pathogens where their primary role is detoxification of NO produced by the immune defense of the host. Although NORs belong to the heme-copper oxidase superfamily, comprising proton-pumping O-2-reducing enzymes, the best studied NORs, cNORs (cytochrome c-dependent), are non-electrogenic. Here, we focus on another type of NOR, qNOR (quinol-dependent). Recombinant qNOR from Neisseria meningitidis, a human pathogen, purified from Escherichia coli, showed high catalytic activity and spectroscopic properties largely similar to cNORs. However, in contrast to cNOR, liposome-reconstituted qNOR showed respiratory control ratios above two, indicating that NO reduction by qNOR was electrogenic. Further, we determined a 4.5 angstrom crystal structure of the N. meningitidis qNOR, allowing exploration of a potential proton transfer pathway from the cytoplasm by mutagenesis. Most mutations had little effect on the activity, however the E-498 variants were largely inactive, while the corresponding substitution in cNOR was previously shown not to induce significant effects. We thus suggest that, contrary to cNOR, the N. meningitidis qNOR uses cytoplasmic protons for NO reduction. Our results allow possible routes for protons to be discussed.

Place, publisher, year, edition, pages
2018. Vol. 8, article id 3637
Keywords [en]
Oxidoreductases, X-ray crystallography
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Biological Sciences
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URN: urn:nbn:se:su:diva-154847DOI: 10.1038/s41598-018-21804-0ISI: 000426045700058PubMedID: 29483528OAI: oai:DiVA.org:su-154847DiVA, id: diva2:1195866
Available from: 2018-04-06 Created: 2018-04-06 Last updated: 2022-09-15Bibliographically approved

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Gonska, NathalieYoung, DavidÄdelroth, Pia

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