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Promotion of lipid storage rather than of thermogenic competence by fetal versus newborn calf serum in primary cultures of brown adipocytes
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för molekylär biovetenskap, Wenner-Grens institut.ORCID-id: 0000-0001-8044-5410
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för molekylär biovetenskap, Wenner-Grens institut.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för molekylär biovetenskap, Wenner-Grens institut.ORCID-id: 0000-0003-2070-1587
Rekke forfattare: 32018 (engelsk)Inngår i: Adipocyte, ISSN 2162-3945, E-ISSN 2162-397X, Vol. 7, nr 3, s. 166-179Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Much current understanding of brown adipocyte development comes from in-vitro cell models. Serum type may affect the behavior of cultured cells and thus conclusions drawn. Here, we investigate effects of serum type (fetal bovine versus newborn calf) on responses to differentiation inducers (the PPAR agonist rosiglitazone or the neurotransmitter norepinephrine) in cultured primary brown adipocytes. Lipid storage was enhanced by fetal versus newborn serum. However, molecular adipose conversion (Pparg2 and Fabp4 expression) was not affected by serum type. Rosiglitazone-induced (7-days) expression of thermogenic genes (i.e. Ucp1, Pgc1a, Dio2 and Elovl3) was not systematically affected by serum type. However, importantly, acute (2h) norepinephrine-induced thermogenic gene expression was overall markedly higher (and adipose genes somewhat lower) in cells cultured in newborn serum. Thus, newborn serum promotes thermogenic competence, and the use of fetal serum in brown adipocyte cultures (as is often routine) counteracts adequate differentiation. Agents that counteract this inhibition may therefore confoundingly be ascribed genuine thermogenic competence-inducing properties.

sted, utgiver, år, opplag, sider
2018. Vol. 7, nr 3, s. 166-179
Emneord [en]
brown adipocytes, thermogenesis, norepinephrine, UCP1, adipose conversion, differentiation, fetal bovine serum, newborn calf serum
HSV kategori
Identifikatorer
URN: urn:nbn:se:su:diva-162064DOI: 10.1080/21623945.2018.1479578ISI: 000448600700003PubMedID: 29912625OAI: oai:DiVA.org:su-162064DiVA, id: diva2:1266267
Tilgjengelig fra: 2018-11-27 Laget: 2018-11-27 Sist oppdatert: 2022-03-23bibliografisk kontrollert

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de Jong, Jasper M. A.Cannon, BarbaraNedergaard, Jan

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