Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Open-tubular columns for liquid and supercritical fluid chromatography
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
1992 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Methods have been developed for the preparation of narrow-bore open tubularcolumns. In this content, condensed gases (butane, chloroethane and Freon114) were used as solvents for the static coating of these columns. Adevice for filling the narrow-bore open tubular columns with solutions incondensed gases has been designed.

The effect of repeated cross-linking of SE-54 stationary phase, usingazo-rerr.-butane as radical initiator, on the chromatographic properties ofthe columns has been studied. Excessive repetition of the immobilizationadversely affected the chromatographic properties. Optimal conditions forthe immobilization of Carbowax 20M stationary phase using Desmodur L75 andDesmodur N75 as cross-linking reagents have been determined.

Properties of immobilized Carbowax 20M stationary phase have beeninvestigated under LC and GC conditions at room and moderately increasedtemperatures. Glass transition temperature of the stationary phase has beenestimated. Significant changes in stationary phase selectivity anddiffusivity, dependent on the reagent applied for stationary phasecross-linking, have been found at temperatures below the glass transitiontemperature. Swelling of the immobilized stationary phase film with water -methanol mobile phases has been studied at different temperatures. Theeffect of the degree of swelling on the column retention and efficiency hasbeen estimated. Significant increase in diffusion coefficients in thestationary phase swollen with methanol at room temperature has beenobserved.

Improvements have been made of an apparatus for measurement of diffusioncoefficients in supercritical carbon dioxide, with regard to the injection,the separation of a solute from a solvent and the back-pressure device. Anoptimization procedure has been used for the estimation of diffusioncoefficients of some test solutes.

A selective ion-exchange stationary phase has been synthesized in situ innarrow-bore columns and, after loading with silver ions, used forargentation supercritical fluid chromatography. Good selectivity towardsunsaturated compounds has been demonstrated.

Place, publisher, year, edition, pages
Stockholm: Stockholm University, 1992. , p. 41
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-163256Libris ID: 7610789ISBN: 91-7153-042-8 (print)OAI: oai:DiVA.org:su-163256DiVA, id: diva2:1272400
Public defence
1992-06-01, Hörsal G, Biologilaboratoriet, Stockholm, 10:00
Note

Härtill 8 uppsatser

Available from: 2018-12-19 Created: 2018-12-19 Last updated: 2018-12-19Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

PDF (Not accessible to users outside Sweden)
By organisation
Department of Analytical Chemistry
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 36 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf