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Physical Properties of Bacterial Outer Membrane Models: Neutron Reflectometry & Molecular Simulation
Stockholm University, Faculty of Science, Department of Organic Chemistry.
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Number of Authors: 62019 (English)In: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 116, no 6, p. 1095-1104Article in journal (Refereed) Published
Abstract [en]

The outer membrane (OM) of Gram-negative bacteria is an asymmetric bilayer having phospholipids in the inner leaflet and lipopolysaccharides in the outer leaflet. This unique asymmetry and the complex carbohydrates in lipopolysaccharides make it a daunting task to study the asymmetrical OM structure and dynamics, its interactions with OM proteins, and its roles in translocation of substrates, including antibiotics. In this study, we combine neutron reflectometry and molecular simulation to explore the physical properties of OM mimetics. There is excellent agreement between experiment and simulation, allowing experimental testing of the conclusions from simulations studies and also atomistic interpretation of the behavior of experimental model systems, such as the degree of lipid asymmetry, the lipid component (tail, head, and sugar) profiles along the bilayer normal, and lateral packing (i.e., average surface area per lipid). Therefore, the combination of both approaches provides a powerful new means to explore the biological and biophysical behavior of the bacterial OM.

Place, publisher, year, edition, pages
2019. Vol. 116, no 6, p. 1095-1104
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Biological Sciences Chemical Sciences
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URN: urn:nbn:se:su:diva-167585DOI: 10.1016/j.bpj.2019.02.001ISI: 000461645500013PubMedID: 30850116OAI: oai:DiVA.org:su-167585DiVA, id: diva2:1301635
Available from: 2019-04-02 Created: 2019-04-02 Last updated: 2019-04-02Bibliographically approved

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