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Solvent-Assisted Paper Spray Ionization Mass Spectrometry (SAPSI-MS) for the Analysis of Biomolecules and Biofluids
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry. University of Parma, Italy.ORCID iD: 0000-0002-0789-3812
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry.ORCID iD: 0000-0002-3167-3772
Stockholm University, Faculty of Science, Department of Environmental Science and Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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Number of Authors: 72019 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 9, article id 10296Article in journal (Refereed) Published
Abstract [en]

Paper Spray Ionization (PSI) is commonly applied for the analysis of small molecules, including drugs, metabolites, and pesticides in biological fluids, due to its high versatility, simplicity, and low costs. In this study, a new setup called Solvent Assisted Paper Spray Ionization (SAPSI), able to increase data acquisition time, signal stability, and repeatability, is proposed to overcome common PSI drawbacks. The setup relies on an integrated solution to provide ionization potential and constant solvent flow to the paper tip. Specifically, the ion source was connected to the instrument fluidics along with the voltage supply systems, ensuring a close control over the ionization conditions. SAPSI was successfully applied for the analysis of different classes of biomolecules: amyloidogenic peptides, proteins, and N-glycans. The prolonged analysis time allowed real-time monitoring of processes taking places on the paper tip, such as amyloid peptides aggregation and disaggregation phenomena. The enhanced signal stability allowed to discriminate protein species characterized by different post translational modifications and adducts with electrophilic compounds, both in aqueous solutions and in biofluids, such as serum and cerebrospinal fluid, without any sample pretreatment. In the next future, application to clinical relevant modifications, could lead to the development of quick and cost-effective diagnostic tools.

Place, publisher, year, edition, pages
2019. Vol. 9, article id 10296
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Chemical Sciences Biological Sciences
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URN: urn:nbn:se:su:diva-171727DOI: 10.1038/s41598-019-45358-xISI: 000475560800001PubMedID: 31311939OAI: oai:DiVA.org:su-171727DiVA, id: diva2:1350044
Available from: 2019-09-10 Created: 2019-09-10 Last updated: 2022-09-15Bibliographically approved

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Riboni, NicolóQuaranta, AlessandroMotwani, HiteshÖsterlund, NicklesGräslund, AstridIlag, Leopold L.

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