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In Vitro Antigenotoxic, Antihelminthic and Antioxidant Potentials Based on the Extracted Metabolites from Lichen,Candelariella vitellina
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute. Jiangsu University, China; Al-Rayan Colleges, Saudi Arabia; Uppsala University, Sweden.
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Number of Authors: 82020 (English)In: Pharmaceutics, ISSN 1999-4923, E-ISSN 1999-4923, Vol. 12, no 5, article id 477Article in journal (Refereed) Published
Abstract [en]

Lichens have recently received great attention due to their pharmacological potentials. The antigenotoxic potential ofC. vitellinaextract (25 and 50 mu g/mL) was assessed in normal human peripheral blood lymphocytes (HPBL) against Mitomycin C (MMC) co-treatments. Flow cytometric analyses of cell cycle distribution, as well as apoptosis (Annexin V/PI), revealed that the extract had significantly (p <= 0.05) ameliorated the MMC toxicity by reducing the apoptotic cells and normalized the cell cycle phases.C. vitellinaexhibited antigenotoxicity by ameliorating the diminished mitotic index and DNA single-strand breaks caused by MMC. Herein, the hydromethanolic extract (80%) ofCandelariella vitellina(Japan) lichen, exhibited very low cytotoxicity towards normal human peripheral lymphocytes (HPBL) with IC50>1000 mu g/mL. In order to explore the antihelminthic effect,Echinococcus granulosusprotoscoleces were used in vitro. Eosin staining revealed significant (p <= 0.05) dose and time-dependent scolicidal effects of the extract confirmed by degenerative alterations as observed by electron scan microscopy. Furthermore, primary and secondary metabolites were investigated using GC-MS and qualitative HPLC, revealing the presence of sugars, alcohols, different phenolic acids and light flavonoids. Significant antioxidant capacities were also demonstrated by DPPH radical-scavenging assay. In conclusion, the promising antigenotoxic, antihelminthic and antioxidant potentials ofC. vitellinaextract encourage further studies to evaluate its possible therapeutic potency.

Place, publisher, year, edition, pages
2020. Vol. 12, no 5, article id 477
Keywords [en]
antihelminthic, antioxidant, apoptosis, Candelariella vitellinametabolites, DNA damage, HPLC, GC-MS
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Chemical Sciences Biological Sciences Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:su:diva-183565DOI: 10.3390/pharmaceutics12050477ISI: 000543393700061PubMedID: 32456266OAI: oai:DiVA.org:su-183565DiVA, id: diva2:1455575
Available from: 2020-07-27 Created: 2020-07-27 Last updated: 2022-03-23Bibliographically approved

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El-Garawani, IslamElkhateeb, WaillEl-Seedi, HeshamKhalifa, Shaden

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