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Current status and future opportunities for serial crystallography at MAX IV Laboratory
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.ORCID iD: 0000-0002-8081-181X
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Number of Authors: 202020 (English)In: Journal of Synchrotron Radiation, ISSN 0909-0495, E-ISSN 1600-5775, Vol. 27, p. 1095-1102Article in journal (Refereed) Published
Abstract [en]

Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports - silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.

Place, publisher, year, edition, pages
2020. Vol. 27, p. 1095-1102
Keywords [en]
serial crystallography, macromolecular crystallography, sample delivery, high-viscosity injectors
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-185298DOI: 10.1107/S1600577520008735ISI: 000562741000001PubMedID: 32876583OAI: oai:DiVA.org:su-185298DiVA, id: diva2:1478046
Available from: 2020-10-21 Created: 2020-10-21 Last updated: 2022-02-25Bibliographically approved

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Shilova, AnastasyaLebrette, HugoFriel, Ross J.Högbom, Martin

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