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Spc1 regulates the signal peptidase-mediated processing of membrane proteins
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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Number of Authors: 62021 (English)In: Journal of Cell Science, ISSN 0021-9533, E-ISSN 1477-9137, Vol. 134, no 13, article id jcs258936Article in journal (Refereed) Published
Abstract [en]

Signal peptidase (SPase) cleaves the signal sequences (SSs) of secretory precursors. It contains an evolutionarily conserved membrane protein subunit, Spc1, that is dispensable for the catalytic activity of SPase and whose role remains unknown. In this study, we investigated the function of yeast Spc1. First, we set up an in vivo SPase cleavage assay using variants of the secretory protein carboxypeptidase Y (CPY) with SSs modified in the N-terminal and hydrophobic core regions. When comparing the SS cleavage efficiencies of these variants in cells with or without Spc1, we found that signal-anchored sequences became more susceptible to cleavage by SPase without Spc1. Furthermore, SPase-mediated processing of model membrane proteins was enhanced in the absence of Spc1 and was reduced upon overexpression of Spc1. Spc1 co-immunoprecipitated with proteins carrying uncleaved signal-anchored or transmembrane (TM) segments. Taken together, these results suggest that Spc1 protects TM segments from SPase action, thereby sharpening SPase substrate selection and acting as a negative regulator of the SPase-mediated processing of membrane proteins.

Place, publisher, year, edition, pages
2021. Vol. 134, no 13, article id jcs258936
Keywords [en]
SPCS1, Signal peptidase, Signal sequence, Spc1, Transmembrane
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-197345DOI: 10.1242/jcs.258936ISI: 000679478500017PubMedID: 34125229OAI: oai:DiVA.org:su-197345DiVA, id: diva2:1599375
Available from: 2021-09-30 Created: 2021-09-30 Last updated: 2022-02-25Bibliographically approved

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Nilsson, IngMarie

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