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Isothermal microcalorimetry measures UCP1-mediated thermogenesis in mature brite adipocytes
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.ORCID iD: 0000-0001-6459-8679
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute. INSERM/Université Paul Sabatier, France.
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Number of Authors: 102021 (English)In: Communications Biology, E-ISSN 2399-3642, Vol. 4, no 1, article id 1108Article in journal (Refereed) Published
Abstract [en]

The activation of thermogenesis in adipose tissue has emerged as an important target for the development of novel anti-obesity therapies. Using multi-well isothermal microcalorimetry, we have demonstrated that mature murine brown and brite adipocytes produce quantifiable heat upon β3-AR stimulation, independently of any anaerobic mechanisms. Additionally, in brite adipocytes lacking UCP1 protein, β3-AR stimulation still induces heat production, albeit to a much lower extent than in their wildtype counterparts, suggesting that UCP1 is an essential component of adrenergic induced thermogenesis in murine brite adipocytes exvivo. Similarly, we could observe an increase in heat production in human-derived adipocytes (hMADS) upon β-AR stimulation. Collectively, these results establish the use of isothermal microcalorimetry as a sensitive and accurate technique for measuring thermogenic responses in intact mature brite adipocytes from murine and human origin.

Place, publisher, year, edition, pages
2021. Vol. 4, no 1, article id 1108
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Biological Sciences
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URN: urn:nbn:se:su:diva-198698DOI: 10.1038/s42003-021-02639-4ISI: 000697665400003PubMedID: 34548622OAI: oai:DiVA.org:su-198698DiVA, id: diva2:1611426
Available from: 2021-11-15 Created: 2021-11-15 Last updated: 2022-02-28Bibliographically approved

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Bokhari, Muhammad HamzaHalleskog, CarinaÅslund, AliceBoulet, NathalieCasadesús Rendos, Evade Jong, Jasper Martin AntonCsikasz, RobertShabalina, IrinaBengtsson, Tore

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Bokhari, Muhammad HamzaHalleskog, CarinaÅslund, AliceBoulet, NathalieCasadesús Rendos, Evade Jong, Jasper Martin AntonCsikasz, RobertShabalina, IrinaBengtsson, Tore
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Department of Molecular Biosciences, The Wenner-Gren Institute
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