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Epidermal 1-O-acylceramides appear with the establishment of the water permeability barrier in mice and are produced by maturating keratinocytes
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Rekke forfattare: 202022 (engelsk)Inngår i: Lipids, ISSN 0024-4201, E-ISSN 1558-9307, Vol. 57, nr 3, s. 183-195Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

1-O-Acylceramides (1-OACs) have a fatty acid esterified to the 1-hydroxyl of the sphingosine head group of the ceramide, and recently we identified these lipids as natural components of human and mouse epidermis. Here we show epidermal 1-OACs arise shortly before birth during the establishment of the water permeability barrier in mice. Fractionation of human epidermis indicates 1-OACs concentrate in the stratum corneum. During in vitro maturation into reconstructed human epidermis, human keratinocytes dramatically increase 1-OAC levels indicating they are one source of epidermal 1-OACs. In search of potential enzymes responsible for 1-OAC synthesis in vivo, we analyzed mutant mice with deficiencies of ceramide synthases (Cers2, Cers3, or Cers4), diacylglycerol acyltransferases (Dgat1 or Dgat2), elongase of very long fatty acids 3 (Elovl3), lecithin cholesterol acyltransferase (Lcat), stearoyl-CoA desaturase 1 (Scd1), or acidic ceramidase (Asah1). Overall levels of 1-OACs did not decrease in any mouse model. In Cers3 and Dgat2-deficient epidermis they even increased in correlation with deficient skin barrier function. Dagt2 deficiency reshapes 1-OAC synthesis with an increase in 1-OACs with N-linked non-hydroxylated fatty acids and a 60% decrease compared to control in levels of 1-OACs with N-linked hydroxylated palmitate. As none of the single enzyme deficiencies we examined resulted in a lack of 1-OACs, we conclude that either there is functional redundancy in forming 1-OAC and more than one enzyme is involved, and/or an unknown acyltransferase of the epidermis performs the final step of 1-OAC synthesis, the implications of which are discussed. 

sted, utgiver, år, opplag, sider
2022. Vol. 57, nr 3, s. 183-195
Emneord [en]
acyl, acyltransferase, Asah, brain, ceramides, Dga1p, Dgat2, epidermis, Farber disease, heart, kidney thymus, Lcat, liver, Lpla2, Lro1p, lung, lymph node, mammals, skin barrier, spleen
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Identifikatorer
URN: urn:nbn:se:su:diva-203475DOI: 10.1002/lipd.12342ISI: 000771878600001PubMedID: 35318678Scopus ID: 2-s2.0-85126876239OAI: oai:DiVA.org:su-203475DiVA, id: diva2:1649228
Tilgjengelig fra: 2022-04-04 Laget: 2022-04-04 Sist oppdatert: 2022-06-08bibliografisk kontrollert

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