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13C- and 15N-labeling of amyloid-β and inhibitory peptides to study their interaction via nanoscale infrared spectroscopy
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.ORCID iD: 0000-0002-7544-092X
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Number of Authors: 92023 (English)In: Communications Chemistry, E-ISSN 2399-3669, Vol. 6, no 1, article id 163Article in journal (Refereed) Published
Abstract [en]

Interactions between molecules are fundamental in biology. They occur also between amyloidogenic peptides or proteins that are associated with different amyloid diseases, which makes it important to study the mutual influence of two polypeptides on each other's properties in mixed samples. However, addressing this research question with imaging techniques faces the challenge to distinguish different polypeptides without adding artificial probes for detection. Here, we show that nanoscale infrared spectroscopy in combination with C-13, N-15-labeling solves this problem. We studied aggregated amyloid-& beta; peptide (A & beta;) and its interaction with an inhibitory peptide (NCAM1-PrP) using scattering-type scanning near-field optical microscopy. Although having similar secondary structure, labeled and unlabeled peptides could be distinguished by comparing optical phase images taken at wavenumbers characteristic for either the labeled or the unlabeled peptide. NCAM1-PrP seems to be able to associate with or to dissolve existing A & beta; fibrils because pure A & beta; fibrils were not detected after mixing. Interactions of proteins or polypeptides with different secondary structures can be studied in a mixture by nanoscale infrared spectroscopy, however, this technique remains challenging for polypeptides with similar secondary structures. Here, the authors demonstrate clear discrimination of two polypeptides from a mixture by scattering-type scanning near-field optical microscopy when one of the components is labeled with C-13- and N-15-isotopes.

Place, publisher, year, edition, pages
2023. Vol. 6, no 1, article id 163
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Chemical Sciences Biochemistry and Molecular Biology
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URN: urn:nbn:se:su:diva-221129DOI: 10.1038/s42004-023-00955-wISI: 001042052900001PubMedID: 37537303Scopus ID: 2-s2.0-85167397298OAI: oai:DiVA.org:su-221129DiVA, id: diva2:1798201
Available from: 2023-09-18 Created: 2023-09-18 Last updated: 2023-09-18Bibliographically approved

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Paul, SumanJenistova, AdelaVosough, FarazBerntsson, ElinaJarvet, JüriGräslund, AstridBarth, Andreas

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