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Direct Competition of ATCUN Peptides with Human Serum Albumin for Copper(II) Ions Determined by LC-ICP MS
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Tallinn University of Technology, Estonia.ORCID iD: 0000-0002-7544-092X
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.ORCID iD: 0000-0001-6836-5610
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Number of Authors: 62023 (English)In: ACS Omega, E-ISSN 2470-1343, Vol. 8, no 37, p. 33912-33919Article in journal (Refereed) Published
Abstract [en]

Copper is an indispensable biometal, primarily serving as a redox-competent cofactor in numerous proteins. Apart from preformed copper-binding sites within the protein structures, small peptide motifs exist called ATCUN, which are composed of an N-terminal tripeptide XZH, able to bind Cu(II) ions in exchangeable form. These motifs are common for serum albumin, but they are also present in a wide range of proteins and peptides. These proteins and peptides can be involved in copper metabolism, and copper ions can affect their biological role. The distribution of copper between the ATCUN peptides, including truncated amyloid-β (Aβ) peptides Aβ4–42 and Aβ11–42, which may be involved in Alzheimer’s disease pathogenesis, is mainly determined by their concentrations and relative Cu(II)-binding affinities. The Cu(II)-binding affinity (log Kd) of several ATCUN peptides, determined by different methods and authors, varies by more than three orders of magnitude. This variation may be attributed to the chemical properties of peptides but can also be influenced by the differences in methods and experimental conditions used for the determination of Kd. In the current study, we performed direct competition experiments between selected ATCUN peptides and HSA by using an LC-ICP MS-based approach. We demonstrated that ATCUN and truncated Aβ peptides Aβ4–16 and Aβ11–15 bind Cu(II) ions with an affinity similar to that for HSA. Our results demonstrate that ATCUN motifs cannot compete with excess HSA for the binding of Cu(II) ions in the blood and cerebrospinal fluid. 

Place, publisher, year, edition, pages
2023. Vol. 8, no 37, p. 33912-33919
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:su:diva-223038DOI: 10.1021/acsomega.3c04649ISI: 001070106300001Scopus ID: 2-s2.0-85173171732OAI: oai:DiVA.org:su-223038DiVA, id: diva2:1805850
Available from: 2023-10-18 Created: 2023-10-18 Last updated: 2024-03-26Bibliographically approved

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Berntsson, ElinaWärmländer, Sebastian K. T. S.

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