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Selective stimulation of GalR1 and GalR2 in rat substantia gelatinosa reveals a cellular basis for the anti- and pro-nociceptive actions of galanin
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi.ORCID-id: 0000-0001-6107-0844
Vise andre og tillknytning
2008 (svensk)Inngår i: Pain, ISSN 0304-3959, E-ISSN 1872-6623, Vol. 137, nr 1, s. 138-146Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Galanin modulates spinal nociceptive processing by interacting with two receptors, GalR1 and GalR2. The underlying neurophysiological mechanisms were examined by whole-cell recording from identified neurons in the substantia gelatinosa of young adult rats. GalR1 was activated with a 'cocktail' containing the GalR1/2 agonist, AR-M 961 (0.5 mu M), in the presence of the GalR2 antagonist, M871 (1.0-2.5 mu M). GalR2 was activated with the selective agonist, AR-M 1896 (0.5-1.0 mu M). Application of the 'GalR1 agonist cocktail' often activated an inwardly-rectifying conductance in delay firing (excitatory) and tonically firing (inhibitory) neurons. This conductance was not activated by AR-M 1896 which instead decreased or increased an outwardly-rectifying conductance at voltages positive to -70 rnV. Despite this variability in its actions on current-voltage relationships, AR-M 1896 very consistently decreased membrane excitability, as measured by cumulative action potential latency in response to a depolarizing current ramp. This strong GalR2-mediated effect was seen in neurons where membrane conductance was decreased, and where membrane excitability might be predicted to increase. GalR2 was also located presynaptically, as AR-M 1896 increased the interevent interval of spontaneous EPSCs in both delay and tonic cells. By contrast, the 'GalR1 agonist cocktail' had little effect on spontaneous EPSCs, suggesting that presynaptic terminals do not express GalR1. These diverse actions of GalR1 and GalR2 activation on both inhibitory and excitatory neurons are discussed in relation to the known spinal antinociceptive and pro-nociceptive actions of galanin, to the possible association of GalR1 with the inhibitory G-protein, G(i/o) and to report that GalR2 activation suppresses Ca(2+) channel currents.

sted, utgiver, år, opplag, sider
2008. Vol. 137, nr 1, s. 138-146
Emneord [en]
Dorsal horn, Neuropathic pain, Allodynia, Analgesic, Hyperalgesia, Electrophysiology, Patch clamp, Neuropeptide
HSV kategori
Identifikatorer
URN: urn:nbn:se:su:diva-14245DOI: 10.1016/j.pain.2007.08.030ISI: 000257631900016OAI: oai:DiVA.org:su-14245DiVA, id: diva2:180765
Tilgjengelig fra: 2008-06-25 Laget: 2008-06-25 Sist oppdatert: 2018-01-13bibliografisk kontrollert

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