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Regulation of the general stress response sigma factor σT by Lon-mediated proteolysis
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute. Stockholm University, Science for Life Laboratory (SciLifeLab).
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute. Stockholm University, Science for Life Laboratory (SciLifeLab).ORCID iD: 0000-0002-4620-2009
Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute. Stockholm University, Science for Life Laboratory (SciLifeLab).ORCID iD: 0000-0002-4091-4291
Stockholm University, Science for Life Laboratory (SciLifeLab). Stockholm University, Faculty of Science, Department of Molecular Biosciences, The Wenner-Gren Institute.ORCID iD: 0000-0002-1469-4424
Number of Authors: 42023 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 205, no 11Article in journal (Refereed) Published
Abstract [en]

The Lon protease is widely conserved in both prokaryotic and eukaryotic organisms and fulfills important regulatory functions. Nevertheless, the number of identified Lon substrates is limited in most organisms, and the precise role of Lon in regulating these proteins is poorly understood. Here, we describe the α-proteobacterial general stress response sigma factor σT as a novel Lon substrate in Caulobacter crescentus. Based on previously published quantitative proteomics data, we find σT to be a promising putative Lon substrate and confirm a direct role of Lon in degrading σT. We show that Lon contributes to the downregulation of σT abundance under optimal conditions and during recovery from sucrose-induced osmotic stress. Furthermore, the presence of the Lon activity regulator LarA enhances Lon-mediated degradation of σT in vitro and reduces σT levels in vivo indicating a role of LarA in modulating Lon-mediated degradation of σT. Together, our results highlight the importance of Lon during the recovery phase following stress exposure by adjusting the concentrations of critical regulators of stress responses.

Place, publisher, year, edition, pages
2023. Vol. 205, no 11
Keywords [en]
regulated proteolysis, Lon protease, ECF sigma factor, general stress response, Caulobacter crescentus
National Category
Microbiology Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-224280DOI: 10.1128/jb.00228-23ISI: 001099059800001PubMedID: 37930077Scopus ID: 2-s2.0-85179426233OAI: oai:DiVA.org:su-224280DiVA, id: diva2:1820361
Available from: 2023-12-18 Created: 2023-12-18 Last updated: 2024-01-15Bibliographically approved

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Akar, RoyaFink, Matthias J.Omnus, Deike J.Jonas, Kristina

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