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Insulin-like growth factor type 1 upregulates uncoupling protein 3.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi.
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2001 (Engelska)Ingår i: Biochem Biophys Res Commun, ISSN 0006-291X, Vol. 287, nr 5, s. 1105-11Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

In this study the expression of uncoupling protein 3 (UCP3) and its regulation by insulin-like growth factor 1 (IGF-I) and insulin in human neuroblastoma SH-SY5Y cells were characterized. Reverse transcriptase-PCR, Western blot, and immunofluorescence analysis showed that SH-SY5Y cells express UCP3 natively. IGF-I induced a time- and concentration-dependent induction of UCP3 protein reaching a twofold expression after 72 h with 10 nM IGF-I. Extremely high insulin concentrations (860 nM) and 10 nM trIGF-I, a truncated form of IGF-I with the same affinity for the IGF-I receptor as the full-length IGF-I, but with lower activity on the insulin receptor, also upregulated UCP3. We conclude that SH-SY5Y cells express UCP3 natively and that the expression is regulated by IGF-I via the IGF-I receptor. Copyright 2001 Academic Press.

Ort, förlag, år, upplaga, sidor
2001. Vol. 287, nr 5, s. 1105-11
Nyckelord [en]
Carrier Proteins/biosynthesis/*genetics, Diabetes Mellitus; Type 2/metabolism, Diabetic Neuropathies/etiology, Humans, Insulin/pharmacology, Insulin-Like Growth Factor I/*pharmacology, Ion Channels, Mitochondria/metabolism, Mitochondrial Proteins, Nerve Tissue/drug effects/*metabolism, Neuroblastoma, Receptor; IGF Type 1/isolation & purification, Receptor; Insulin/isolation & purification, Tumor Cells; Cultured, Uncoupling Agents/metabolism, Up-Regulation
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Cellbiologi
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URN: urn:nbn:se:su:diva-20503PubMedID: 11587536OAI: oai:DiVA.org:su-20503DiVA, id: diva2:187029
Tillgänglig från: 2009-01-23 Skapad: 2009-01-23 Senast uppdaterad: 2015-03-09Bibliografiskt granskad

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PubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=11587536&dopt=Citation

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Gustafsson, HelenaForsby, Anna
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Institutionen för neurokemi
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