This study evaluates the effect of different diets supplemented with vitamin solutions on Pangasius catfish broodstock reproduction, growth performances, and the survival rates of fish larvae and fingerling. The growth and reproductive performances of breeders fed with different test diets showed significant differences among the six tested diets (p < 0.05). The highest final body weight (FBW), weight gain (WG), daily weight gain (DWG), specific growth rate (SGR) of broodstock, and survival rate of Pangasius fingerlings were found in Treatment 5, which contained 0.6% H-OVN mixed with 12.6% algal oil, and Treatment 3, which contained 0.6% vitamin premix H-OVN. The average gonadosomatic index (GSI), relative fecundity index (RFI), fertilized eggs, hatching rates of eggs, and survival rate of fingerlings was 9.1 ± 2.8 (6.7–12.8%), 133,224 ± 39,090 (104,267–199,512 eggs/kg), 77.9 ± 22.2 (62.2–93.6%), and 45.3 ± 17.4 (22.0–66.3%), respectively. The findings of this study showed that the diet containing 35% CP contents supplemented with 0.6% vitamin premix H-OVN mixed with algal oils showed the highest results in terms of growth, reproductive performance indices, and survival rates of Pangasius catfish fingerlings.