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TRPV1 expression and activity during retinoic acid-induced neuronal differentiation
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi.ORCID-id: 0000-0001-6662-0868
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi.ORCID-id: 0000-0001-6298-201X
2009 (Engelska)Ingår i: Neurochemistry International, ISSN 0197-0186, E-ISSN 1872-9754, Vol. 55, nr 8, s. 768-774Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The transient receptor potential vanilloid subtype 1 (TRPV1) is a Ca2+-permeable channel primarily expressed in dorsal root ganglion neurons. Besides its function in thermogenic nociception and neurogenic inflammation, TRPV1 is involved in cell migration, cytoskeleton reorganisation and in neuronal guidance.  To explore the TRPV1 level and activity during conditions for neuronal maturation, TRPV1-expressing SHSY5Y neuroblastoma cells were differentiated into a neuronal phenotype using all-trans-retinoic acid (RA). We show that RA highly up-regulated the total and cell surface TRPV1 protein expression but the TRPV1 mRNA level was unaffected. The up-regulated receptors were localised to the cell bodies and the developed neurites. Furthermore, RA increased both the basal intracellular free Ca2+ concentration by 30 % as well as the relative capsaicin-induced Ca2+ influx. The results show that TRPV1 protein expression increases during RA-induced differentiation in vitro, which generates an altered intracellular Ca2+ homeostasis.

Ort, förlag, år, upplaga, sidor
2009. Vol. 55, nr 8, s. 768-774
Nationell ämneskategori
Biologiska vetenskaper Kemi
Identifikatorer
URN: urn:nbn:se:su:diva-30177DOI: 10.1016/j.neuint.2009.07.011ISI: 000271690000008OAI: oai:DiVA.org:su-30177DiVA, id: diva2:241951
Tillgänglig från: 2009-10-06 Skapad: 2009-10-06 Senast uppdaterad: 2017-12-13Bibliografiskt granskad
Ingår i avhandling
1. Activation and Regulation of TRPV1: Studies in Recombinant Human Neuroblastoma TRPV1-SHSY5Y Cells
Öppna denna publikation i ny flik eller fönster >>Activation and Regulation of TRPV1: Studies in Recombinant Human Neuroblastoma TRPV1-SHSY5Y Cells
2009 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

TRPV1 is a transmembrane non-selective cation channel with preference for Ca2+. The receptor is primarily localised on dorsal root ganglion neurons and is activated by numerous endogenous and exogenous potentially irritating ligands eliciting pain. The TRPV1 expression and activity are regulated by several neurotrophic agents and inflammatory mediators via activation of phosphorylation cascades.

In this thesis a stably TRPV1-expressing cell clone of the human neuroblastoma cell line SHSY5Y was established with the purpose to study regulation of TRPV1 through a straightforward and reproducible approach.

In paper I it is reported that the neurotrophic factors insulin, and IGF-1 up-regulate TRPV1 in the TRPV1-SHSY5Y cells. Additionally, the involved signalling pathways are suggested. This is of interest because both TRPV1 activity and expression is altered in diabetic patients with painful neuropathies, and so is insulin and IGF-1 signalling.

Results from paper II show that the neuronally differentiating morphogen retinoic acid (RA) increases TRPV1 protein levels and TRPV1-mediated Ca2+ influxes. Furthermore, the basal Ca2+ level is increased after RA treatment in TRPV1-SHSY5Y cells but not in native SHSY5Y cells. The TRPV1-SHSY5Y cells also develop into a more mature neuronal phenotype than the native SHSY5Y cells after six days of RA-induced differentiation. Hence, TRPV1 might be involved in neurogenesis.

In paper III-IV it is concluded that the TRPV1-SHSY5Y cells can be used in a semi-high throughput screening (HTS)-mode to adress TRPV1-mediated Ca2+ influxes. In this assay it is shown that anionic linear aliphatic surfactants might be potent ligands of TRPV1. As a concluding remark, the TRPV1–SHSY5Y cells can be utilised to assess activation and regulation of TRPV1 in an easy-to-use and robust model system.

Ort, förlag, år, upplaga, sidor
Stockholm: Department of Neurochemistry, Stockholm University, 2009. s. 91
Nyckelord
nociception, neurotrophic factors, eye irritation, stable transfection, Ca2+
Nationell ämneskategori
Neurovetenskaper
Forskningsämne
neurokemi med molekylär neurobiologi
Identifikatorer
urn:nbn:se:su:diva-30182 (URN)978-91-7155-954-8 (ISBN)
Disputation
2009-11-13, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 13:00 (Engelska)
Opponent
Handledare
Anmärkning
At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 3: In press. Paper 4: In press.Tillgänglig från: 2009-10-22 Skapad: 2009-10-06 Senast uppdaterad: 2018-01-13Bibliografiskt granskad

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EL Andaloussi-Lilja, JohannaLundqvist, JessicaForsby, Anna
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