Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Quantifying Colocalization by Correlation: The Pearson Correlation Coefficient is Superior to the Mander's Overlap Coefficient
Stockholms universitet, Naturvetenskapliga fakulteten, Wenner-Grens institut.
Stockholms universitet, Naturvetenskapliga fakulteten, Wenner-Grens institut.
2010 (Engelska)Ingår i: CYTOMETRY PART A, ISSN 1552-4922, Vol. 77A, nr 8, s. 733-742Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The Pearson correlation coefficient (PCC) and the Mander's overlap coefficient (MOC) are used to quantify the degree of colocalization between fluorophores. The MOC was introduced to overcome perceived problems with the PCC. The two coefficients are mathematically similar, differing in the use of either the absolute intensities (MOC) or of the deviation from the mean (PCC). A range of correlated datasets, which extend to the limits of the PCC, only evoked a limited response from the MOC. The PCC is unaffected by changes to the offset while the MOC increases when the offset is positive. Both coefficients are independent of gain. The MOC is a confusing hybrid measurement, that combines correlation with a heavily weighted form of co-occurrence, favors high intensity combinations, downplays combinations in which either or both intensities are low and ignores blank pixels. The PCC only measures correlation. A surprising finding was that the addition of a second uncorrelated population can substantially increase the measured correlation, demonstrating the importance of excluding background pixels. Overall, since the MOC is unresponsive to substantial changes in the data and is hard to interpret, it is neither an alternative to nor a useful substitute for the PCC. The MOC is not suitable for making measurements of colocalization either by correlation or co-occurrence.

Ort, förlag, år, upplaga, sidor
2010. Vol. 77A, nr 8, s. 733-742
Nyckelord [en]
correlation, colocalization, co-occurrence, Mander's overlap coefficient, Pearson correlation coefficient
Identifikatorer
URN: urn:nbn:se:su:diva-49195DOI: 10.1002/cyto.a.20896ISI: 000280432500005OAI: oai:DiVA.org:su-49195DiVA, id: diva2:377138
Anmärkning
authorCount :2Tillgänglig från: 2010-12-13 Skapad: 2010-12-13 Senast uppdaterad: 2022-02-24Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltext

Person

Parmryd, Ingela

Sök vidare i DiVA

Av författaren/redaktören
Parmryd, Ingela
Av organisationen
Wenner-Grens institut

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 281 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf