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Dual functions of the human antimicrobial peptide LL-37-Target membrane perturbation and host cell cargo delivery
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
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2010 (Engelska)Ingår i: Biochimica et Biophysica Acta - Biomembranes, ISSN 0005-2736, E-ISSN 1879-2642, Vol. 1798, nr 12, s. 2201-2208Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The mechanisms behind target vs. host cell recognition of the human antimicrobial peptide LL-37 remain ill-defined. Here, we have investigated the membrane disruption capacity of LL-37 using large unilamellar vesicles (LUVs) composed of varying mixtures of POPC, POPG and cholesterol to mimic target and host membranes respectively. We show that LL-37 is unable to induce leakage of entrapped calcein from zwitterionic POPC LUVs, whereas leakage from LUVs partially composed of POPG is fast and efficient. In accordance with typical antimicrobial peptide behavior, cholesterol diminished LL-37 induced leakage. By using linear dichroism and flow oriented LUVs, we found that LL-37 orients with the axis of its induced alpha-helix parallel to the membrane surface in POPC:POPG (7:3) LUVs. In the same system, we also observed a time-dependent increase of the parallel alpha-helix LD signal on timescales corresponding to the leakage kinetics. The increased LD may be connected to a peptide translocation step, giving rise to mass balance across the membrane. This could end the leakage process before it is complete, similar to what we have observed. Confocal microscopy studies of eukaryotic cells show that LL-37 is able to mediate the cell delivery of non-covalently linked fluorescent oligonucleotides, in agreement with earlier studies on delivery of plasmid DNA (Sandgren et al., J. Biol. Chem. 279 (2004) 17951). These observations highlight the potential dual functions of LL-37 as an antimicrobial agent against bacterial target cells and a cell-penetrating peptide that can deliver nucleic acids into the host cells.

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2010. Vol. 1798, nr 12, s. 2201-2208
Nyckelord [en]
LL-37, Antimicrobial peptide, Calcein leakage, CD, LD, Confocal microscopy, Heparan sulphate
Nationell ämneskategori
Biokemi och molekylärbiologi Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:su:diva-51335DOI: 10.1016/j.bbamem.2009.12.011ISI: 000284392900004OAI: oai:DiVA.org:su-51335DiVA, id: diva2:385049
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authorCount :7

Tillgänglig från: 2011-01-11 Skapad: 2011-01-10 Senast uppdaterad: 2017-12-11Bibliografiskt granskad

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Gräslund, Astrid
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Institutionen för biokemi och biofysik
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Biochimica et Biophysica Acta - Biomembranes
Biokemi och molekylärbiologiBiokemi och molekylärbiologi

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