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The B-WICH chromatin-remodelling complex facilitates the binding of c-Myc and histone acetyl transferases and regulates RNA pol III transcription
Stockholms universitet, Naturvetenskapliga fakulteten, Wenner-Grens institut, Avdelningen för cellbiologi.
Stockholms universitet, Naturvetenskapliga fakulteten, Wenner-Grens institut, Avdelningen för cellbiologi.
Stockholms universitet, Naturvetenskapliga fakulteten, Wenner-Grens institut, Avdelningen för cellbiologi.
Stockholms universitet, Naturvetenskapliga fakulteten, Wenner-Grens institut, Avdelningen för cellbiologi.
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
Abstract [en]

Transcription of the 5S rRNA genes and 7SL genes by RNA polymerase III is necessary for cell growth and proliferation. The chromatin-remodelling complex B-WICH is associated with these genes, and siRNA-silencing of one component, the WSTF protein, reduces the level of transcription. However, the molecular mechanism is unclear. We show here that the role of B-WICH is to promote the binding of RNA polymerase III and RNA polymerase III factors, TFIIIA, TFIIIB and TFIIIC. WSTF knock down by siRNA resulted in a decreased recruitment of these initiation factors and, consequently, RNA polymerase III, to promoters. In addition, B-WICH induced a local alteration of the chromatin structure around the 5S rRNA and 7SL RNA genes, leading to a reduced acetylation of histone H3, in particular H3K9-Ac. A reduction in the level of WSTF also caused a loss of c-myc binding to the genes. We propose a model in which B-WICH complex is required to maintain an open chromatin structure around these RNA polymerase III genes, a prerequisite for other factors to associate at the gene.

Emneord [en]
B-WICH, chromatin-remodelling, c-Myc, polymerase III, transcription
HSV kategori
Forskningsprogram
cellbiologi
Identifikatorer
URN: urn:nbn:se:su:diva-75269OAI: oai:DiVA.org:su-75269DiVA, id: diva2:515407
Tilgjengelig fra: 2012-04-12 Laget: 2012-04-12 Sist oppdatert: 2016-01-29bibliografisk kontrollert
Inngår i avhandling
1. Regulation of RNA polymerase I and RNA polymerase III transcription by the chromatin remodelling complex B-WICH
Åpne denne publikasjonen i ny fane eller vindu >>Regulation of RNA polymerase I and RNA polymerase III transcription by the chromatin remodelling complex B-WICH
2012 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Ribosomal biogenesis is an important process which determines the rate of cell growth and is involved in cell response to proliferation, differentiation, cellular nutritional state and stress. The chromatin remodelling complex B-WICH composed of WSTF, SNF2h and NM1 is involved in transcription by the RNA pol I and RNA pol III. In this study I investigated the mechanism by which the B-WICH complex modulates the RNA pol I and RNA pol III transcription. I showed that B-WICH binds to the 45S genes, 5S rRNA and 7SL RNA genes, and remodels the chromatin. The remodelling at the 45S genes occurs at the promoter, leading higher accessibility to histone acetyltransferases, such as PCAF and p300. In the RNA pol III transcription, the chromatin outside of the gene is more open, leading to binding of c-Myc, with the subsequent recruitment of histone acetylation resulting in H3-Ac. The importance of the chromatin remodelling around the genes was particularly clear in WSTF knock-down cells, in which the binding of RNA pol III and auxiliary transcription factors at the 5S rRNA and 7SL RNA gene promoters were totally abolished. I concluded that B-WICH functions in a similar manner on both RNA pol I and RNA pol III genes, remodels chromatin locally at the promoter and around the genes, which allows other factors to bind. I also investigated the role of B-WICH in the control of RNA pol I transcription, in the cell cycle and in response to glucose/energy status. My results showed that the B-WICH complex disassembled in prophase, and reassembled at G1. WSTF is hyperphosphorylated in mitosis, and with the dephosphorylation at the end of telophase, the SNF2h and NM1 bind to the WSTF. A reduction of the association of the B-WICH complex is seen in cells treated with inhibitors of different signalling pathways. Furthermore, during glucose deprivation, the level of B-WICH decreases at the RNA pol I promoter. These results demonstrate that the chromatin remodelling complex B-WICH is important in the transcription of RNA pol I and RNA pol III genes, as maintaining the chromatin state in an active configuration. 

sted, utgiver, år, opplag, sider
Stockholm: The Wenner-Gren Institute, Stockholm University, 2012. s. 47
Emneord
B-WICH, Chromatin remodelling, ribosomal genes, transcription
HSV kategori
Forskningsprogram
cellbiologi
Identifikatorer
urn:nbn:se:su:diva-75204 (URN)978-91-7447-513-5 (ISBN)
Disputas
2012-05-11, lecture room E306, Arrheniuslaboratorierna, Svante Arrhenius väg 20 C, Stockholm, 13:00 (engelsk)
Opponent
Veileder
Merknad

At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 2: Manuscript. Paper 3: Submitted.

Tilgjengelig fra: 2012-04-19 Laget: 2012-04-11 Sist oppdatert: 2017-03-08bibliografisk kontrollert

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