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Separation of Lipid Classes by HPLC on a Cyanopropyl Column
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.ORCID-id: 0000-0003-2666-0704
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.
2012 (engelsk)Inngår i: Lipids, ISSN 0024-4201, E-ISSN 1558-9307, Vol. 47, nr 1, s. 93-99Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A new method for the separation and identification of lipid classes by normal-phase HPLC on a cyanopropyl column is described. The use of a simple binary gradient, with toluene as a component, provided a rapid separation of non-polar as well as phospholipid classes. The inherent small differences in performances between possible non-polar eluent components of the gradient, such as hexane, heptane, and iso-octane, had a pronounced impact on retention times for individual phospholipid classes. Separation of molecular species within a lipid class could also be observed.

sted, utgiver, år, opplag, sider
2012. Vol. 47, nr 1, s. 93-99
Emneord [en]
Cyanopropyl column, Normal-phase HPLC, Lipid classes, Phospholipids, Toluene, Hexane, Heptane, Iso-octane
HSV kategori
Identifikatorer
URN: urn:nbn:se:su:diva-76743DOI: 10.1007/s11745-011-3627-0ISI: 000299532400010OAI: oai:DiVA.org:su-76743DiVA, id: diva2:527211
Tilgjengelig fra: 2012-05-18 Laget: 2012-05-16 Sist oppdatert: 2022-02-24bibliografisk kontrollert
Inngår i avhandling
1. Essentially Lipids: Analytical methods for the characterization of lipid materials
Åpne denne publikasjonen i ny fane eller vindu >>Essentially Lipids: Analytical methods for the characterization of lipid materials
2015 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

This thesis describes analytical methods for chromatographic characterizations of lipids in biological and technical systems.

Lipids are a group of compounds with a central role in all known forms of life. In addition to the biological roles, lipids are also components in many products of our daily usage. The application areas include food, pharma, cosmetics, biofuels, etc.

Analytical characterization of lipids is challenging since a typical lipid extract often contains several hundred unique compounds which also could vary significantly in chemical properties. Separation is in many cases crucial for identification and quantification of individual lipids and here high performance liquid chromatography (HPLC) is regarded as the technique of first choice today.

In the present work, methodologies for both normal phase (NP) and reversed phase (RP) HPLC are presented. The focus has been to develop methods that are fast, have low analytical complexity and are compatible with several detection systems. Cyanopropyl coated silica was evaluated as stationary phase for NP-HPLC with the aim to separate all the lipid classes in common lipid extracts. The analytes were eluted with a binary mobile phase system based on hexane, toluene and methanol which yielded in a total runtime of 30 minutes. In a subsequent study, additional stationary phases for NP-HPLC were evaluated for online sample cleanup of polycyclic aromatic hydrocarbons (PAHs) in lipid matrixes. In the demonstrated methodology for RP-HPLC, a binary system consisting of methanol/water was utilized with phenylpropyl coated silica to elute all tested lipid classes in 30 minutes.

The methodologies were compatible with various detection techniques including evaporative light scattering detection (ELSD), charged aerosol detection (CAD) as well as electrospray mass spectrometry (ESI-MS) and were applied to characterize lipid materials of both biological and technical nature. 

sted, utgiver, år, opplag, sider
Stockholm: Department of Environmental Science and Analytical Chemistry, Stockholm Univeristy, 2015. s. 49
Emneord
Lipids, Chromatography, HPLC, Charged Aerosol Detection, Evaporative light scattering detection, mass spectrometry
HSV kategori
Forskningsprogram
analytisk kemi
Identifikatorer
urn:nbn:se:su:diva-113052 (URN)978-91-7649-077-8 (ISBN)
Disputas
2015-02-27, Magnélisalen, Kemiska Övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 10:00 (svensk)
Opponent
Veileder
Tilgjengelig fra: 2015-02-05 Laget: 2015-01-22 Sist oppdatert: 2022-02-23bibliografisk kontrollert

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