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micMicroextraction by packed sorbent and liquid chromatography-tandem mass spectrometry as a tool for quantification of peptides in plasma samples: determination of sensory neuron-specific receptors agonist BAM8-22 and antagonist BAM22-8 in plasma samples
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.ORCID-id: 0000-0002-3862-8878
2013 (engelsk)Inngår i: BMC Biomedical chromotography, ISSN 0269-3879, E-ISSN 1099-0801, Vol. 27, nr 3, s. 396-403Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Microextraction by packed sorbent (MEPS) is a miniaturized, solid-phase extraction (SPE) technique that works online with gas chromatography (GC) and liquid chromatography (LC). Not only is the automation process with MEPS advantageous, but the much smaller volumes of the samples, solvents and dead space in the system also provide other significant advantages such as the speed and the simplicity of the sample preparation process. In this study MEPS has been evaluated for quantification of sensory neuron-specific receptors agonist (BAM8-22). Owing to the instability of BAMs, the focus was on fast extraction and determination of the peptide online using LC-MS/MS. Sorbents such as C2, C8 and ENV+ (hydroxylated polystyrenedivinylbenzene copolymer) were investigated in the present study. MEPS-C8 gave the best results compared with C2 and ENV and it was used for the method validation. The calibration curve was obtained within the concentration range of 20.03045nmol/L in plasma. The regression correlation coefficients for plasma samples were 0.99 for all runs (n=6). The between-batch accuracy and precision for BAM8-22 ranged from 13 to 2.0% and from 4.0 to 14%, respectively. Additionally, the accuracy and precision for BAM22-8 ranged from 13 to 7.0% and from 3.0 to 12%, respectively. The present method was used for pharmacokinetic studies for BAMs in plasma samples.

sted, utgiver, år, opplag, sider
2013. Vol. 27, nr 3, s. 396-403
Emneord [en]
BAM peptides, plasma samples, microextraction by packed sorbent, liquid chromatography, mass spectrometry
HSV kategori
Identifikatorer
URN: urn:nbn:se:su:diva-88289DOI: 10.1002/bmc.2806ISI: 000314471700019OAI: oai:DiVA.org:su-88289DiVA, id: diva2:610699
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AuthorCount:3;

Tilgjengelig fra: 2013-03-12 Laget: 2013-03-12 Sist oppdatert: 2019-12-09bibliografisk kontrollert

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