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Evaluation of microextraction by packed sorbent and micro-liquid chromatography-tandem mass spectrometry as a green approach in bioanalysis
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för analytisk kemi.ORCID-id: 0000-0002-3862-8878
2013 (engelsk)Inngår i: BMC Biomedical chromotography, ISSN 0269-3879, E-ISSN 1099-0801, Vol. 27, nr 10, s. 1225-1233Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

In this study the use of micro-liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was investigated in routine bioanalysis application for separation and quantification of pro-drug AZD6319 (developed for aldezheimer treatment). Microextraction by packed sorbent (MEPS) was used as sample clean-up method. The focus of this study was put on the evaluation of the usability of smaller column diameters such as 1.0 and 0.3mm instead of 2.1mm in bioanalysis application to reduce solvent consumption and sample volumes. Solvent consumption was reduced by 80% when a 1.0mm column was used compared with 2.1mm column. Robustness of the micro-columns in terms of accuracy and precision was investigated. The application of LC-MS/MS for the quantitative analysis of AZD6319 in plasma samples showed good selectivity, accuracy and precision. The coefficients of determination (R-2) were >0.998 for all runs using plasma samples on the studied micro-columns. The inter-day accuracy values for quality control samples ranged from 99 to 103% and from 96 to 105% for 0.3x50mm and 1.0x50mm columns, respectively. The inter-day precision values ranged from 4.0 to 9.0% and from 4.0 to 8.0% for 0.3x50 and 1.0x50mm columns, respectively. In addition the sensitivity was increased by three times using a 1.0mm column compared with 2.1mm. Furthermore, robustness of the micro-columns from different manufacturers was investigated.

sted, utgiver, år, opplag, sider
2013. Vol. 27, nr 10, s. 1225-1233
Emneord [en]
microextraction by packed sorbent, micro-LC columns, plasma samples, green bioanalysis
HSV kategori
Identifikatorer
URN: urn:nbn:se:su:diva-94018DOI: 10.1002/bmc.2839ISI: 000323900600001OAI: oai:DiVA.org:su-94018DiVA, id: diva2:651846
Merknad

AuthorCount:2;

Tilgjengelig fra: 2013-09-27 Laget: 2013-09-24 Sist oppdatert: 2019-12-09bibliografisk kontrollert

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