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Single Mutations That Redirect Internal Proton Transfer in the ba(3) Oxidase from Thermus thermophilus
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
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2013 (Engelska)Ingår i: Biochemistry, ISSN 0006-2960, E-ISSN 1520-4995, Vol. 52, nr 40, s. 7022-7030Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The ba(3)-type cytochrome c oxidase from Thermus thermophilus is a membrane-bound proton pump. Results from earlier studies have shown that with the aa(3)-type oxidases proton uptake to the catalytic site and pump site occurs simultaneously. However, with ba(3) oxidase the pump site is loaded before proton transfer to the catalytic site because the proton transfer to the latter is slower than that with the aa(3) oxidases. In addition, the timing of formation and decay of catalytic intermediates is different in the two types of oxidases. In the present study, we have investigated two mutant ba(3) CytcOs in which residues of the proton pathway leading to the catalytic site as well as the pump site were exchanged, Thr312Val and Tyr244Phe. Even though ba(3) CytcO uses only a single proton pathway for transfer of the substrate and pumped protons, the amino-acid residue substitutions had distinctly different effects on the kinetics of proton transfer to the catalytic site and the pump site. The results indicate that the rates of these reactions can be modified independently by replacement of single residues within the proton pathway. Furthermore, the data suggest that the Thr312Val and Tyr244Phe mutations interfere with a structural rearrangement in the proton pathway that is rate limiting for proton transfer to the catalytic site.

Ort, förlag, år, upplaga, sidor
American Chemical Society (ACS), 2013. Vol. 52, nr 40, s. 7022-7030
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:su:diva-97038DOI: 10.1021/bi4008726ISI: 000326355500010OAI: oai:DiVA.org:su-97038DiVA, id: diva2:670476
Anmärkning

AuthorCount:6;

Funding Agencies:

Swedish Research Council;  National Institutes of Health HL 16101;

Stockholm University  

Tillgänglig från: 2013-12-03 Skapad: 2013-12-02 Senast uppdaterad: 2022-02-24Bibliografiskt granskad

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Smirnova, Irinavon Ballmoos, ChristophAdelroth, PiaBrzezinski, Peter

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Smirnova, Irinavon Ballmoos, ChristophAdelroth, PiaBrzezinski, Peter
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Biochemistry
Biokemi och molekylärbiologi

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