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Different preparation techniques – similar results? On the quality of thin-ground sections of archaeological bone
Stockholms universitet, Humanistiska fakulteten, Institutionen för arkeologi och antikens kultur, Osteoarkeologiska forskningslaboratoriet.
Stockholms universitet, Humanistiska fakulteten, Institutionen för arkeologi och antikens kultur, Osteoarkeologiska forskningslaboratoriet.
2015 (engelsk)Inngår i: International journal of osteoarchaeology, ISSN 1047-482X, E-ISSN 1099-1212, Vol. 25, nr 6, s. 935-945Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Palaeohistology as a valuable diagnostic instrument is dependent on the production of high-quality thin-ground sections from dry bone. The objective of this study was to consider technical differences and assess the qualitative outcomes of five techniques for preparing thin-ground sections from dry archaeological bone. Established techniques with long follow-up times and excellently documented results were compared with simpler and cheaper time-saving techniques. Evaluations were made of the quality of thin sections obtained by one classical machine-based embedding technique, two revised versions of the same technique, one manual moulding technique based on Frost's rapid technique and one manual hybrid technique. Five osteological specimens of differing quality were prepared following the manuals for these five techniques and examined microscopically with respect to a list of standardised histological and diagenetic parameters. Alterations in the specimens attributable to preparation effects were recorded, and observations were scored with reference to three criteria: section quality, technical quality and staining. The results show that embedding techniques are to prefer. Superglue should not be used as a mounting or embedding medium. Manual grinding comes with several limitations, and machine cutting and grinding are preferred. Haematoxylin staining can be successfully applied to embedded specimens, giving more information on microscopic diagenetic processes. A stepwise manual for a revision of the classical embedding technique is presented. The time required for producing sections using classical embedding techniques is shortened from 6 weeks to 3.7 days by refining the preparation/polymerization processes involved with no loss of osteological data.

sted, utgiver, år, opplag, sider
2015. Vol. 25, nr 6, s. 935-945
Emneord [en]
dry bone, histology, microscopy, moulding, embedding, manual/machine technique
HSV kategori
Forskningsprogram
osteoarkeologi
Identifikatorer
URN: urn:nbn:se:su:diva-112725DOI: 10.1002/oa.2382ISI: 000368455400014OAI: oai:DiVA.org:su-112725DiVA, id: diva2:780195
Tilgjengelig fra: 2015-01-14 Laget: 2015-01-14 Sist oppdatert: 2017-12-05bibliografisk kontrollert

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