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CPP-Based Delivery System for In Vivo Gene Delivery
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för neurokemi. Tartu University, Estonia.
2015 (engelsk)Inngår i: Cell-Penetrating Peptides: Methods and Protocols / [ed] Ülo Langel, Springer-Verlag New York, 2015, Vol. 1324, s. 339-347Kapittel i bok, del av antologi (Fagfellevurdert)
Abstract [en]

The method presents most important steps in estimating a CPP-mediated reporter gene delivery in a mouse model. The method is applicable for administrating noncovalent CPP/pDNA complexes via i.v. injection and for analysis of luciferase levels in tissue homogenates. This method could be extended to analyze the delivery of different nucleic acid cargos with other types of delivery vectors. First, a simple method is presented for assessing the stability of complexes in blood after i.v. administration, based on quantitation of a fluorescently labeled nucleic acid. Secondly, a protocol is presented for assessing and analyzing luciferase levels in mouse organs.

sted, utgiver, år, opplag, sider
Springer-Verlag New York, 2015. Vol. 1324, s. 339-347
Serie
Methods in Molecular Biology, ISSN 1064-3745 ; 1324
HSV kategori
Identifikatorer
URN: urn:nbn:se:su:diva-124908DOI: 10.1007/978-1-4939-2806-4_22ISI: 000376037700023PubMedID: 26202280ISBN: 978-1-4939-2805-7 (tryckt)ISBN: 978-1-4939-2806-4 (tryckt)OAI: oai:DiVA.org:su-124908DiVA, id: diva2:891238
Tilgjengelig fra: 2016-01-06 Laget: 2016-01-06 Sist oppdatert: 2016-06-20bibliografisk kontrollert

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