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Ionic Strength Modulation of the Free Energy Landscape of A beta(40) Peptide Fibril Formation
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik. The National Institute of Chemical Physics and Biophysics, Estonia.
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.ORCID-id: 0000-0001-5784-7673
Stockholms universitet, Naturvetenskapliga fakulteten, Institutionen för biokemi och biofysik.
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Antal upphovsmän: 52016 (Engelska)Ingår i: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 138, nr 21, s. 6893-6902Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Protein misfolding and formation of cross-beta structured amyloid fibrils are linked to, many neurodegenerative disorders. Although recently developed,quantitative approaches have started to reveal the molecular nature of self-assembly and fibril formation of proteins and peptides, it is yet unclear how these self-organization events are precisely modulated by microenvironmental factors, which are known to strongly affect the macroscopic aggregation properties. Here, we characterize the explicit effect of ionic strength on the microscopic aggregation rates of amyloid beta peptide (A beta 40) self-association, implicated in Alzheimer's disease. We found that physiological ionic strength accelerates A beta 40 aggregation kinetics by promoting surface-catalyzed secondary nucleation reactions. This promoted catalytic effect can be assigned to shielding of electrostatic repulsion between Monomers on the fibril surface or between the fibril surface itself and monomeric peptides. Furthermore, we observe the formation of two different beta-structured states with =similar but distinct spectroscopic features, which can be assigned to an off-pathway immature state (F-beta*) and a mature stable State (F-beta), where salt favors formation of the F-beta fibril morphology. Addition of salt to preformed F-beta* accelerates transition to F-beta, underlining the dynamic nature of A beta 40 fibrils in solution. On the basis of,these results we suggest a model where salt decreases the free-energy barrier for A beta 40 folding to the F-beta state, favoring the buildup of the mature fibril morphology while omitting competing, energetically less favorable structural states.

Ort, förlag, år, upplaga, sidor
2016. Vol. 138, nr 21, s. 6893-6902
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Kemi
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URN: urn:nbn:se:su:diva-131935DOI: 10.1021/jacs.6b04511ISI: 000377151400029PubMedID: 27171340OAI: oai:DiVA.org:su-131935DiVA, id: diva2:946418
Tillgänglig från: 2016-07-05 Skapad: 2016-07-04 Senast uppdaterad: 2020-03-04Bibliografiskt granskad

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Abelein, AxelJarvet, JüriBarth, AndreasGräslund, AstridDanielsson, Jens
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Institutionen för biokemi och biofysik
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Journal of the American Chemical Society
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