Change search
ReferencesLink to record
Permanent link

Direct link
FtsZ does not initiate membrane constriction at the onset of division
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Number of Authors: 3
2016 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 6, 33138Article in journal (Refereed) Published
Abstract [en]

The source of constriction required for division of a bacterial cell remains enigmatic. FtsZ is widely believed to be a key player, because in vitro experiments indicate that it can deform liposomes when membrane tethered. However in vivo evidence for such a role has remained elusive as it has been challenging to distinguish the contribution of FtsZ from that of peptidoglycan-ingrowth. To differentiate between these two possibilities we studied the early stages of division in Escherichia coli, when FtsZ is present at the division site but peptidoglycan synthesizing enzymes such as FtsI and FtsN are not. Our approach was to use correlative cryo-fluorescence and cryo-electron microscopy (cryo-CLEM) to monitor the localization of fluorescently labeled FtsZ, FtsI or FtsN correlated with the septal ultra-structural geometry in the same cell. We noted that the presence of FtsZ at the division septum is not sufficient to deform membranes. This observation suggests that, although FtsZ can provide a constrictive force, the force is not substantial at the onset of division. Conversely, the presence of FtsN always correlated with membrane invagination, indicating that allosteric activation of peptidoglycan ingrowth is the trigger for constriction of the cell envelope during cell division in E. coli.

Place, publisher, year, edition, pages
2016. Vol. 6, 33138
National Category
Other Natural Sciences
URN: urn:nbn:se:su:diva-134224DOI: 10.1038/srep33138ISI: 000382861900001PubMedID: 27609565OAI: diva2:1033594
Available from: 2016-10-07 Created: 2016-10-03 Last updated: 2016-10-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Daley, Daniel O.
By organisation
Department of Biochemistry and Biophysics
In the same journal
Scientific Reports
Other Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

ReferencesLink to record
Permanent link

Direct link