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The role of nuclear membrane proteins in differentiation and chromatin organization
Stockholm University, Faculty of Science, Department of Neurochemistry.ORCID iD: 0000-0002-5556-7966
2016 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

The nuclear envelope, consisting of an outer and an inner nuclear membrane, surrounds the genomic material. The genomic material (chromatin) is highly structured with (transcriptionally inactive) heterochromatin mostly found in the nuclear periphery and (transcriptionally active) euchromatin mostly found in the nuclear interior. Underlying the nuclear envelope is the nuclear lamina that consists of lamin proteins and nuclear envelope transmembrane proteins (NETs), which organize chromatin in the nuclear periphery. There are several hundred uncharacterized tissue-specific NETs, with only a few linked to cellular differentiation. Induced pluripotent stem cells (iPSCs) enable studies of early differentiation and are a promising tool for cell replacement therapies.

In this licentiate thesis, we have focused on investigating the role of the inner nuclear membrane protein Samp1 in chromatin organization and cell differentiation. Overexpression of Samp1 induced a fast differentiation of iPSCs, suggesting that Samp1 may be involved in the differentiation process. We have also developed a novel image analysis method to be able to monitor chromatin organization in live cells. Depletion of Samp1 affected chromatin distribution and resulted in increased formation of peripheral heterochromatin, contradictory to what is expected of other characterized NETs. It is possible that Samp1 might have a role in both differentiation and chromatin organization and that future studies might link these two processes together.

Place, publisher, year, edition, pages
Stockholm: US-AB , 2016. , 36 p.
Keyword [en]
nuclear membrane proteins, chromatin organization, epigenetics, differentiation, stem cells
National Category
Cell Biology Other Chemistry Topics
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
URN: urn:nbn:se:su:diva-135773ISBN: 978-91-7649-632-9OAI: oai:DiVA.org:su-135773DiVA: diva2:1048950
Presentation
2016-12-20, Heilbronnsalen, C458, Svante Arrhenius väg 16B, Stockholm, 13:00 (English)
Opponent
Supervisors
Available from: 2016-11-23 Created: 2016-11-22 Last updated: 2016-11-23Bibliographically approved
List of papers
1. An inner nuclear membrane protein induces rapid differentiation of human induced pluripotent stem cells
Open this publication in new window or tab >>An inner nuclear membrane protein induces rapid differentiation of human induced pluripotent stem cells
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(English)Manuscript (preprint) (Other academic)
National Category
Other Chemistry Topics Cell Biology
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
urn:nbn:se:su:diva-135805 (URN)
Funder
Swedish Research CouncilSwedish Cancer Society
Available from: 2016-11-23 Created: 2016-11-23 Last updated: 2016-11-28
2. Monitoring of the epigenetic state in live cells
Open this publication in new window or tab >>Monitoring of the epigenetic state in live cells
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(English)Manuscript (preprint) (Other academic)
Keyword
epigenetics
National Category
Other Chemistry Topics Cell Biology
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
urn:nbn:se:su:diva-135806 (URN)
Funder
Swedish Research CouncilSwedish Cancer Society
Available from: 2016-11-23 Created: 2016-11-23 Last updated: 2016-11-23

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Bergqvist, Cecilia
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