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Energetics of side-chain snorkeling in transmembrane helices probed by nonproteinogenic amino acids
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics. Stockholm University, Science for Life Laboratory (SciLifeLab).
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Number of Authors: 6
2016 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 113, no 38, 10559-10564 p.Article in journal (Refereed) Published
Abstract [en]

Cotranslational translocon-mediated insertion of membrane proteins into the endoplasmic reticulum is a key process in membrane protein biogenesis. Although the mechanism is understood in outline, quantitative data on the energetics of the process is scarce. Here, we have measured the effect on membrane integration efficiency of nonproteinogenic analogs of the positively charged amino acids arginine and lysine incorporated into model transmembrane segments. We provide estimates of the influence on the apparent free energy of membrane integration (Delta G(app)) of snorkeling of charged amino acids toward the lipid-water interface, and of charge neutralization. We further determine the effect of fluorine atoms and backbone hydrogen bonds (H-bonds) on Delta G(app). These results help establish a quantitative basis for our understanding of membrane protein assembly in eukaryotic cells.

Place, publisher, year, edition, pages
2016. Vol. 113, no 38, 10559-10564 p.
Keyword [en]
membrane protein, nonproteinogenic amino acids, translocon
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-135178DOI: 10.1073/pnas.1606776113ISI: 000383622600041PubMedID: 27601675OAI: oai:DiVA.org:su-135178DiVA: diva2:1049434
Available from: 2016-11-24 Created: 2016-11-01 Last updated: 2016-11-24Bibliographically approved

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Lara, PatriciaLindahl, Erikvon Heijne, Gunnar
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Department of Biochemistry and BiophysicsScience for Life Laboratory (SciLifeLab)
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