RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
Number of Authors: 32
2017 (English)In: International Journal of Radiation Biology, ISSN 0955-3002, E-ISSN 1362-3095, Vol. 93, no 1, 36-47 p.Article in journal (Refereed) Published
Purpose: In the framework of the 'Realizing the European Network of Biodosimetry' (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures. Results: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points (<= 0.94 Gy). For higher dose points (>= 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method. Conclusion: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories.
Place, publisher, year, edition, pages
2017. Vol. 93, no 1, 36-47 p.
Biodosimetry, micronucleus assay, triage, radiation accident, RENEB
Biological Sciences Mechanical Engineering Radiology, Nuclear Medicine and Medical Imaging
IdentifiersURN: urn:nbn:se:su:diva-140232DOI: 10.1080/09553002.2016.1206231ISI: 000392891400006PubMedID: 27673504OAI: oai:DiVA.org:su-140232DiVA: diva2:1078831