Distribution of local anesthetics between aqueous and liposome phases
Number of Authors: 8
2017 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1479, 194-203 p.Article in journal (Refereed) Published
Liposomes were used as biomimetic models in capillary electrokinetic chromatography (EKC) for the determination of distribution constants (K-D) of certain local anesthetics and a commonly used preservative. Synthetic liposomes comprised phosphatidylcholine and phosphatidylglycerol phospholipids with and without cholesterol. In addition, ghost liposomes made from red blood cell (RBC) lipid extracts were used as pseudostationary phase to acquire information on how the liposome composition affects the interactions between anesthetics and liposomes. These results were compared with theoretical distribution coefficients at pH 7.4. In addition to 25 degrees C, the distribution constants were determined at 37 and 42 degrees C to simulate physiological conditions. Moreover, the usability of five electroosmotic flow markers in liposome (LEKC) and micellar EKC (MEKC) was studied. LEKC was proven to be a convenient and fast technique for obtaining data about the distribution constants of local anesthetics between liposome and aqueous phase. RBC liposomes can be utilized for more representative model of cellular membranes, and the results indicate that the distribution constants of the anesthetics are greatly dependent on the used liposome composition and the amount of cholesterol, while the effect of temperature on the distribution constants is less significant.
Place, publisher, year, edition, pages
2017. Vol. 1479, 194-203 p.
Liposome electrokinetic chromatography., Red blood cell lipids, Distribution constants, EOF markers, Local anesthetics, Cholesterol
Biological Sciences Chemical Sciences
IdentifiersURN: urn:nbn:se:su:diva-140220DOI: 10.1016/j.chroma.2016.12.005ISI: 000392681300022PubMedID: 27955893OAI: oai:DiVA.org:su-140220DiVA: diva2:1079008
40th International Symposium on Capillary Chromatography and 13th GC × GC Symposium (RIVA 2016), Riva del Garda, Italy, 29 May – 3 June 2016.