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Cell-penetrating peptides recruit type A scavenger receptors to the plasma membrane for cellular delivery of nucleic acids
Stockholm University, Faculty of Science, Department of Neurochemistry. University of Tartu, Estonia.
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Number of Authors: 5
2017 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 31, no 3, 975-988 p.Article in journal (Refereed) Published
Abstract [en]

Scavenger receptors (SRs) are a large family of multifunctional receptors that are involved in a range of physiologic and pathologic processes. The ability of class A scavenger receptors (SR-As) to bind anionic ligands facilitates the internalization of negatively charged cell-penetrating peptide (CPP)-nucleic acid nanocomplexes and thus makes them attractive targets for delivery of various nucleic acids. Recently, we demonstrated that SR-A3 and SR-A5 are recruited from intracellular membranes to the plasma membrane after incubation with PepFect 14-splice-switching oligonucleotide complexes. Here, we examined the mechanisms responsible for translocation of SR-As to the cell surface. We demonstrate that, in addition to nanocomplexes, some amphipathic CPPs are able to induce externalization of SR-A3 and SR-A5, and this process requires the presence of calcium ions. Furthermore, translocation of SR-A3 and SR-A5 requires activity of phosphatidylinositol-3-kinase, intact actin cytoskeleton, and the presence of serum proteins in culture medium.

Place, publisher, year, edition, pages
2017. Vol. 31, no 3, 975-988 p.
Keyword [en]
SR-A3, SR-A5, Ca2+ signaling, PI3K
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-142695DOI: 10.1096/fj.201600811RISI: 000395671200014PubMedID: 27881484OAI: oai:DiVA.org:su-142695DiVA: diva2:1093734
Available from: 2017-05-08 Created: 2017-05-08 Last updated: 2017-05-08Bibliographically approved

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